Page 86 - B.Tech Biotech Curriculum and Syllabus R2017 - REC

Department of BIOTECHNOLOGY, REC

TEXT BOOKS
1. Fawatt, H.H. and Wood, W.S., ―Safety and Accident Prevention in Chemical Operation―, Wiley
Interscience, 1965.
2. Marcel, V.C., Major Chemical Hazard- Ellis Harwood Ltd., Chi Chester, UK, 1987.
3. Skeleton, B., Process Safety Analysis : An introduction, Institution of chemical Engineers, U.K., 1997.
4. Hyatt, N., Guidelines for process hazards analysis, hazards identification & risk analysis,bDyadem Press,
2004.

REFERENCES
1. Handley, W., ―Industrial Safety Hand Book ―, 2nd Edn., McGraw-Hill Book Company,1969.
2. Heinrich, H.W. Dan Peterson, P.E. and Rood, N., ―Industrial Accident Prevention―, McGraw-Hill Book
Co., 1980.
3. Chemical Process Safety: Fundamentals with Applications, Daniel A. Crowl, J.F. Louvar, Prentice Hall,
NJ, 1990.
4. Taylor, J.R., Risk analysis for process plant, pipelines and transport, Chapman and Hall, London, 1994

BT17E78 ADVANCED IMMUNOTECHNOLOGY L T P C
3 0 0 3

OBJECTIVES:
 To impart knowledge about the development of immune cells and their function
 To provide knowledge on immune defence mechanism, through which pathogenelimination occurs
 To explain the principle of various immunological techniques
 To provide basic knowledge on various types of vaccines and their development
 To enable the student to lay a strong foundation on immunological oriented research.

UNIT I INTRODUCTION 12
Cells of the immune system and their development; primary and secondary lymphoid organs; humoral and
cell mediated immune responses; complement activation pathway.

UNIT II ANTIBODIESAND IMMUNO TECHNIQUES 10
Monoclonal antibodies and their use in diagnostics; ELISA; Agglutination tests; Antigen detection assay;
Plaque Forming Cell Assay, western blotting, RIA and immunofluoresence.

UNIT III CELLULAR IMMUNOLOGY 8
PBMC seperation from the blood; identification of lymphocytes based on CD markers; FACS;
Lymphoproliferation assay; Mixed lymphocyte reaction; Cr51 release assay; cytokine bioassays- IL2, gamma
IFN, TNF alpha.; HLA typing.

UNIT IV VACCINE TECHNOLOGY 6
Basic principles of vaccine development; protein based vaccines; DNA vaccines; Plant based vaccines;
recombinant antigens as vaccines; reverse vaccinology

UNIT V IMMUNOTHERAPEUTICS 9
Engineered antibodies; catalytic antibodies; idiotypic antibodies; combinatorial libraries for antibody
isolation, fusion proteins, cellular therapies and immunosuppressive therapy.

TOTAL : 45 PERIODS
COURSE OUTCOMES:
At the end of the course, the students will
 Identify the role of the immune system on elimination of pathogens
 Basic principles of various immunological techniques
 Ability to separate the immune cells based onCD markers and also acquired the ability to perform
cytokine bioassay.

Curriculum and Syllabus | B.Tech. Biotechnology | R2017 Page 86