Page 104 - 2022-23 BIOFACT Catalogue

BioFACT™

OneStep RT-PCR Series ⓗ

Fast and Convenient OneStep RT-PCR Reaction in a Single Tube

BioFACT™ OneStep RT-PCR Series is designed for convenience and high e ciency in carrying out RT-PCR in a single tube at one step.
Included BioFACT™ RTase and BioFACT™ H-Star Taq DNA Polymerase is blended in one mixture with best optimized rate to simplify
reaction step and reducing the possibility of contamination. BioFACT™ OneStep RT-PCR Series has great rst-strand cDNA yields by
its thermostable characteristic at 50℃ for activation of BioFACT™ RTase and it allows robust, high speci c ampli cation by BioFACT™
H-Star Taq DNA Polymerase included in the mixture. It is optimized to amplify less than 1 kb size target.

Feature
• Thermostable and RNase H variant of M-MLV RTase (RNase H ) - • Chemical mediated Hot Start enzyme
• Synthesize cDNA at 42℃ – 50℃ (highest activity at 50℃) • Great e ciency & high speci city
• Synthesis of long length cDNA (＜14 kb) • Easy sample optimization
• Synthesis gene size : < 1 kb PCR • Low-copy transcripts ampli cation
• Synthesis primer : gene speci c primer

Application
• RT-PCR

QC DATA
- Comparison of amplification efficiency on various RNA concentrations between BioFACT™ OneStep RTPCR Kit
and other companies' OneStep RT-PCR Kits

β-Actin (1 kb) BRCA2 (0.7 kb) β-Actin (1 kb) TNFR1 (0.6 kb) BRCA2 (0.4 kb)
M A B C D E F M M A B C D E F M M A B C D M M A B C D M M A B C D M
BIOFACT BIOFACT

Company A Company A

Company B Company B

Target : Human Total RNA PCR Condition : 60°C, 40 cycles Target : Human Total RNA PCR Condition : 60°C, 40 cycles
M : 1kb DNA Ladder M : 1kb DNA Ladder
A : 100 ng B : 20 ng C : 4 ng D : 0.8 ng E : 0.16 ng F : 0.032ng A : 100 ng B : 10 ng C : 1 ng D : 0.1 ng

104 BIOFACT CATALOGUE