Page 108 - 2022-23 BIOFACT Catalogue

BioFACT™

Bst DNA Polymerase

DNA Polymerase for Isothermal Ampli cation

BioFACT™ Bst DNA Polymerase is a recombinant protein lacking the 5'→3' exo-nuclease activity of Bacillus stearothermophilus DNA
Polymerase. The optimum conditions for the product are 65℃, 30 to 60 minutes, and it can be applied to Loop-Mediated isothermal
ampli cation (LAMP) PCR with isothermal ampli cation technology.

Feature
• Synthesis primer : gene speci c primer • Lacking 5´→3´ exonuclease activity
• Suitable for applications requiring thermophilic strand displacement • 5' → 3' exonuclease activity : No
• Optimization activity at 65℃ • 3' → 5' exonuclease activity ( delity) : No
• Relative activity 80~90% at 60℃, 65~75% at 55℃, 15~20% at 50℃

Application
• Loop-mediated isothermal ampli cation(LAMP) • Whole genome ampli cation
• Isothermal ampli cation • RT-LAMP

QC DATA

- Comparison of LAMP PCR activity by concentration of enzyme between BioFACT™ Bst DNA Polymerase and other company’s
polymerase
Company A BIOFACT Company A BIOFACT
M A B C D E F G H M A B C D E F G H M A B C D E F G H M A B C D E F G H

Target : Human Actin Target : Human Chromosome 22
M : 1kb DNA Ladder M : 1kb DNA Ladder
A : 8U B : 4U C : 2U D : 1U E : 0.5U F : 0.25U G : 0.125U H : NTC A : 8U B : 4U C : 2U D : 1U E : 0.5U F : 0.25U G : 0.125U H : NTC

108 BIOFACT CATALOGUE