Page 133 - 2022-23 BIOFACT Catalogue

DNA / RNA Preparation

- DaBead™ Viral RNA/DNA Prep Kit _ APX-32P [VN801A-096]
Sample Magnetic DNA
Bead

1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6
Bead binding Lysis bu er Washing bu er1,2 Elution bu er Release bead

- DaBead™ Viral RNA/DNA Prep Kit _ 96well Press Pipettor [VN901-096, VN401-096]

Cell Lysis & DNA binding Washing DNA elution
precipitation

QC DATA
- Comparison of amplification efficiency in various primer sets between DaBead™ Viral RNA/DNA Prep Kit and
the other company’s viral RNA/DNA extraction kit
[Viral RNA Detection Primer Set-1] [Viral RNA Detection Primer Set-2]
FAM Texas Red FAM Texas Red

10 0 10 0
10 -1 10 -1
10 -2 10 -2
10 -3 10 -3

Column Type 8well Magnetic Bead Pipettor
Viral RNA 10 0 Viral RNA 10 -1 Viral RNA 10 -2 Viral RNA 10 -3
ΔCt value
FAM Texas Red FAM Texas Red FAM Texas Red FAM Texas Red
Primer Company A - Column Type 16.61 16.16 20.50 20.32 24.12 24.49 27.68 28.14
Set-1 8well Magnetic Bead Pipettor 16.14 16.43 19.60 20.03 23.07 23.83 26.77 27.98
Primer Company A - Column Type 16.11 14.94 19.87 18.59 23.88 22.63 26.94 25.65
Set-2 8well Magnetic Bead Pipettor 15.86 14.60 19.52 18.03 22.68 21.50 26.19 24.94
Comparison of the DaBead™ Viral RNA/DNA Prep Kit[Magnetic Bead Type], Campany A [Column Type] for extraction of novel coronavirus(COVID-19). COVID-19 viral RNA was purified from
10-fold diluted in spiked COVID-19 serum and detected by RT-PCR(Viral RNA Detection Primer Set-1/-2) using 1/10 volume of purified RNA solution. Measure from 1 X 10 6 copies using
COVID-19 Viral RNA.
[Viral RNA Detection Primer Set-3]

Viral RNA 10 0 Viral RNA 10 -1 Viral RNA 10 -2 Viral RNA 10 -3

Column Type Semi-Auto Magnetic Bead Type
Comparison of the DaBead™ Viral RNA/DNA Prep Kit[Magnetic Bead Type], HiGene™ Viral RNA/DNA Prep Kit [Column Type] for extraction of novel coronavirus(COVID-19).
COVID-19 viral RNA was purified from serially diluted in spiked COVID-19 serum and detected by RT-PCR(Viral RNA Detection Primer Set-3) using 1/10 volume of purified RNA solution.
Measure from 1 X 10 6 copies using COVID-19 Viral RNA.

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BIOFACT CATALOGUE