Page 134 - 2022-23 BIOFACT Catalogue

QC DATA
- Comparison of amplification efficiency for extracted viral RNA/DNA in different batch products

Lot-1/ Lot-2 / Lot-3
FAM
1 X 10 7 copies 1 X 10 6 copies 1 X 10 5 copies 1 X 10 4 copies 1 X 10 3 copies 1 X 10 2 copies
using Viral RNA using Viral RNA using Viral RNA using Viral RNA using Viral RNA using Viral RNA

Texas Red

Copies No. Copies No.
Copies No. Copies No.
10 7 10 6 10 5 10 4 10 3 10 2 10 7 10 6 10 5 10 4 10 3 10 2
Lot-1 Average Ct value 22.82 26.46 30.06 33.81 36.20 39.18 Lot-1 Average Ct value 22.35 25.88 29.48 33.33 37.04 ND
[FAM] Lot-2 Average Ct value 22.84 26.54 30.15 34.00 36.94 39.65 [Texas Lot-2 Average Ct value 22.02 25.59 29.18 32.97 36.85 ND
Red]
Lot-3 Average Ct value 22.57 26.17 30.00 33.22 36.54 39.37 Lot-3 Average Ct value 21.95 25.52 29.22 32.83 36.50 ND
Standard Deviation 0.153 0.195 0.078 0.404 0.37 0.236 Standard Deviation 0.217 0.196 0.27 0.321 0.797 ND
%CV between Lot 0.67 0.74 0.26 1.20 1.01 0.60 %CV between Lot 0.98 0.76 0.92 0.97 2.16 ND
Lot-to-lot variation from DaBead™ Viral RNA/DNA Prep Kit[Cat. No. VN901-096, BioFACT] . COVID-19 viral RNA was purified from 10-fold diluted in spiked COVID-19 serum
and detected by qRT-PCR (Viral RNA Detection Primer Set-1) using 1/10 volume of purified RNA solution.

- Amplification test of extracted viral RNA/DNA from clinical samples using viral RNA detection primer set-1
(VN901-096, DaBead™ Viral RNA/DNA Prep Kit & SJ1-MPS96, DaBead™ 96well Magnetic Press Pipettor)

Negative Sample

Positive Sample

[FAM] [Texas Red] Internal control

134 BIOFACT CATALOGUE