Page 136 - 2022-23 BIOFACT Catalogue

Column Type
Solution Type
Magnetic Bead Type
Easy Type
HiGene™

Type Viral RNA/DNA Prep Kit
Magnetic Solution Column Easy
Bead Type Type Type
High-Purity Viral RNA/DNA Extraction

HiGene™ Viral RNA/DNA Prep Kit[Column Type] can quickly and conveniently extract RNA from Serum, cell-culture media, cell free
uids sample or plasma. The process does not require the use of organic solvents or ethanol precipitation steps, and is thus ideal
for the safe and convenient extraction. This kit can be used for isolation of vial RNA/DNA from broad of RNA and DNA viruses such
as SARS-COV-2, Adenovirus, Norovirus, Zika virus, HCV and HIV. Procedural directions of HiGene™ Viral RNA/DNA Prep Kit[Column
Type] is very simple; users may purity Viral RNA/DNA from a variety of target sources within 20 minutes.
Feature
• CE-IVD certified • Efficient removal of impurities using non-alcoholic washing buffer
• No carrier RNA required • Glass microfiber membrane column based
• High purity & high yield • Safety use; no phenol & no chloroform

Application
• Quantitative PCR(qPCR, qRT-PCR) • cDNA synthesis
• Pathogen detection • Microarray
• Poly-A selection • Northern blotting

Workflow

- HiGene™ Viral RNA/DNA Prep Kit [VN101-100]

Column binding & Viral RNA/DNA Eluted Viral
Cell lysis
washing elution RNA/DNA

QC DATA
- Comparison of amplification efficiency for extracted viral RNA/DNA using HiGene™ Viral RNA/DNA Prep Kit and the other company’s
viral RNA/DNA extraction kit (qRT-PCR/qPCR)
<Adenovirus (DNA)> <Norovirus (RNA)>
BIOFACT Company Company
∆Ct value
[VN101] A B
Adenovirus 29.2 29.5 29.8
Norovirus 34.8 34.7 35.0
BIOFACT
Company A
Company B

136 BIOFACT CATALOGUE