Page 140 - 2022-23 BIOFACT Catalogue
P. 140

- Comparison of extraction efficiency on various storage periods _ Saliva, Oral Epithelial Cell
                  <Saliva - 500 ㎕>
                   M     1                          2                           3                          4  M
                                                        M : 1kb DNA Ladder
                                                        1: Day 1 (Refrigerated)
                                                        2: Day 2 (Refrigerated)
                                                        3: Day 3 (Refrigerated)
                                                        4: Day 7 (Refrigerated)
                                                        ※ Concentration decreases rapidly over time due to the death of bacteria cells.
                  - Genomic DNA extraction using DaBead™ Magnetic Separation Stand (96 well. 8-strip tube) and DaBead™ 8well Magnetic Bead Pipettor
                  < Whole Blood - 200 ㎕ >     < Gram(-) Becterium - Cultured Cell 1㎖ >  < Oral Epithelial Cell >
                           M                           M                            M



                  M : 1kb DNA Ladder               1% Agarose gel               3㎕ loading               Elution volume : 100 ㎕


                  - Comparison of efficiency by extraction Method between BIOFACT Magnetic Bead type & SDS manual type _ Saliva
                     BIOFACT Magnetic Bead type   M  SDS manual type   BIOFACT Magnetic Bead type   M  SDS manual type




                                      M                                          M





                  M : 1kb DNA Ladder               1% Agarose gel               3㎕ loading               Elution volume : 100 ㎕
                  - Comparison of extraction efficiency between DaBead™ 8well Magnetic Bead Pipettor and automated nucleic acid preparation system
                  <Gram(-) Bacterium>         <Gram(+) Bacterium>          <Whole Blood>
                      Semi-Auto  M    Auto         Semi-Auto  M   Auto         Semi-Auto  M    Auto




                                        10 8  cells                  10 8  cells                 200 ㎕
                  <Beef>                      <Oral Epithelial>
                      Semi-Auto  M    Auto         Semi-Auto  M   Auto

                                                                           Semi-Auto : 8well Magnetic Bead Pipettor
                                                                           Auto : Automated Nucleic Acid Preparation System
                                                                           M : 1kb DNA Ladder
                                        25 mg                              1% Agarose gel     3㎕ loading      Elution volume : 100 ㎕
                  - Extraction efficiency test - DaBead™ 96well Magnetic Bead Press Pipettor
                  < Bacterium >               < Bacterium >
                           M                           M





                                                                           M : 1kb DNA Ladder     1% Agarose gel     3㎕ loading
                                                                           Elution volume : 100 ㎕



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