Page 144 - 2022-23 BIOFACT Catalogue

- DaBead™ Genomic DNA Prep Kit For Plant _ APX-32P [GD803A-096]
Sample Magnetic DNA
Bead

1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6
Bead binding Lysis bu er Washing bu er1,2 Elution bu er Release bead

- DaBead™ Genomic DNA Prep Kit For Plant _ 96well Press Pipettor [GD903-096, GD403-096]

Cell Lysis & DNA binding Washing DNA elution
precipitation

QC DATA
- Comparison of efficiency by extraction method between BIOFACT Magnetic Bead type and column type
<Option>
Spinacia oleracea Oenanthe javanica Aralia cordata Capsella bursa-pastoris Castanea crenata Protocol Option :
(Leaf) (Stem) (Stem) (Root) (Leaf) Increase of yield when
treated with chloroform
Magnetic Magnetic Magnetic Magnetic
M Column Bead M Column Bead M Column Bead M Column Bead M 1 2 3

M : 1kb DNALadder 1: BIOFACT Column Type 2 : BIOFACT Magnetic Bead Type M : 1kb DNALadder 1: BIOFACT Column Type
1% Agarose gel 3㎕ loading Elution volume : 100 ㎕ 2 : BIOFACT Magnetic Bead Type (Chloroform(-))
3 : BIOFACT Magnetic Bead Type (Chloroform(+))

- Comparison of template LOD
Spinacia oleracea Oenanthe javanica Aralia cordata Capsella bursa-pastoris
(Leaf) (Stem) (Stem) (Root)
Spinacia oleracea Oenanthe javanica Aralia cordata Capsella bursa-pastoris
M 1 2 3 4 M 1 2 3 4 M 1 2 3 4 M 1 2 3 4 (Leaf) (Stem) (Stem) (Root)

M : 1kb DNA Ladder 1 : Undiluted (5 0 ) 2 : 5 -1 3 : 5 -2 4 : 5 -3 Target Gene : rpoB Template : Undiluted (5 0 ) , 5 -1 , 5 -2 , 5 -3 Target Gene : 18S rRNA
** As a result of the Template LOD test, PCR can be performed without ** As a result of the Template LOD test, PCR can be performed without
PCR Inhibition (High Purity) PCR Inhibition (High Purity)

144 BIOFACT CATALOGUE