Page 148 - 2022-23 BIOFACT Catalogue
P. 148

Column Type
                                  Solution Type
                   Magnetic Bead Type
                                  Easy Type


                  DaBead™ GMO DNA
             Magnetic       Solution        Column           Easy
             Bead Type        Type           Type            Type
                  Extraction Kit I For Potato, Bean, Maize


                  High-E ciency GMO DNA Extraction using Magnetic Bead






                  DaBead™ Genomic DNA Prep Kit is a kit for extracting GMO DNA using Magnetic Bead. It can extract DNA from various sample
                  samples (Potato, Bean, Maize), and it is easy to use even without prior experience as extraction process is simple and convenient.



                    Feature
                    • Magnetic Bead type                  • Extraction from GMO-modified plant samples
                    • Extraction with a small amount of sample available   • Scale up extraction available
                    • Quick process by minimizing the use of centrifuge    (DaBead™ Magnetic Separation Stand, 1.5 ㎖ type)
                    • Easy to use even without prior experience


                    Application
                    • PCR (Real-Time PCR, Multiplex PCR, Long PCR etc.)  • Construction of clone library
                    • Southern blotting analysis          • Genotyping (SNP, SSR, AFLP, T-RFLP etc.)


                    Workflow

                    - DaBead™ GMO DNA Extraction Kit [GD706-100]






                                                      Magnet

                      Cell Lysis &   Magnetic Bead  Magnetic Bead                   Magnet
                     precipitation  biniding    washing & dry    DNA elution             Eluted DNA



                    QC DATA

                   - Comparison of extraction efficiency on various scales between BIOFACT Magnetic Bead type and column type
                    <Potato>                    <Bean>                       <Maize>
                       0.1g  0.2g  0.3g  0.4g  0.5g  0.1g  0.2g  0.3g  0.4g  0.5g  0.1g  0.2g  0.3g  0.4g  0.5g
                    M  A      B     A      B       A     B     A      B       A     B  M  M  A      B     A      B       A     B     A      B       A     B  M  M  A      B     A      B       A     B     A      B       A     B   M






                    M : 1kb DNA Ladder              A : Column               B : Magnetic Bead  M : 1kb DNA Ladder              A : Column               B : Magnetic Bead  M : 1kb DNA Ladder              A : Column               B : Magnetic Bead
                                                                             1% Agarose gel               3㎕ loading               Elution volume : 100 ㎕



     148     BIOFACT CATALOGUE
   143   144   145   146   147   148   149   150   151   152   153