Page 149 - 2022-23 BIOFACT Catalogue

DNA / RNA Preparation

- Comparison of PCR efficiency on various template concentration between BIOFACT Magnetic Bead type and column type
<Potato> <Bean> <Maize>
M Column M Magnetic Bead M Column M Magnetic Bead M Column M Magnetic Bead

* Primer mixture : rbcL(Plant - chloroplast DNA) * Primer mixture : rpoB(Plant - chloroplast DNA) * Primer mixture : ITS2(Plant - chloroplast DNA)
1 : Undiluted (5 0 ) 2 : 10 -1 3 : 10 -2 4 : 10 -3 5 : 10 -4 6 : 10 -5 1 : Undiluted (5 0 ) 2 : 10 -1 3 : 10 -2 4 : 10 -3 5 : 10 -4 6 : 10 -5 1 : Undiluted (5 0 ) 2 : 10 -1 3 : 10 -2 4 : 10 -3 5 : 10 -4 6 : 10 -5

- Comparison of extraction efficiency according to various sampling methods between BIOFACT Magnetic Bead type and
column type

<Potato - 50 mg> <Bean - 40 mg> <Maize - 100 mg>
Soy Bean Black Bean Red Bean
Liquid Liquid Liquid Liquid
Lump Piece Crushed Grinder nitrogen Grinder nitrogen Grinder nitrogen Grinder nitrogen Grinder Crushed
M A B A B A B A B M M A B A B A B A B A B A B M M A B A B A B M

M : 1kb DNA Ladder A : Column B : Magnetic Bead M : 1kb DNA Ladder A : Column B : Magnetic Bead M : 1kb DNA Ladder A : Column B : Magnetic Bead
1% Agarose gel 3㎕ loading Elution volume : 100 ㎕

- Comparison of extraction efficiency between BIOFACT Magnetic Bead type and automated nucleic acid preparation system

BIOFACT Magnetic Bead Auto BIOFACT Magnetic Bead Auto

1 2 3 4 5 6 M 1 2 3 4 5 6 13 14 15 16 17 18 M 13 14 15 16 17 18

BIOFACT Magnetic Bead Auto BIOFACT Magnetic Bead Auto

7 8 9 10 11 12 M 7 8 9 10 11 12 19 20 21 22 23 24 M 19 20 21 22 23 24

1 : GT73 rapeseed (GMO) 2 : NG rapeseed (NON-GMO) 3 : Carrot seed 4 : Tiny(tomato) 5 : Beotimmog 6 : Berryking 7 : Supersun
8 : Hoyong 9 : Kyupilang 10 : Lovely240 11 : Mailokku 12 : Redpam 13 : Sangcherry 14 : Betatine
15 : Lapito 16 : Shinnuri 17 : Hambak 18 : Shinlovely 19 : Rokkusan 20 : Minichal 21 : Shinshu
22 : Shinlovely 23 : Potato1 24 : Potato2 M : 1kb DNA Ladder

M : 1kb DNA Ladder
BIOFACT Magnetic Bead : 1.5 ㎖ Magnetic stand
Auto : Automated Nucleic Acid Preparation System
1% Agarose gel 3㎕ loading Elution volume : 100 ㎕

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BIOFACT CATALOGUE