Page 34 - 2022-23 BIOFACT Catalogue

BIOFACT DNA Polymerase

Selection GuideⅡ

3' → 5'
5' → 3'
cDNA
Product Exonuclease Exonuclease A-tailing Product Extension Yield Specificity Sensitivity Synthesis Isothermal
Time
Size
(fidelity)
BioFACT™ Real-Time PCR Yes No Yes < 1 kb 1 min / kb ＊＊ ＊＊＊＊＊ ＊＊
BioFACT™ F-Star Taq Yes No Yes < 3 kb 1 min / kb ＊＊＊ ＊＊＊＊ ＊＊＊＊
BioFACT™ Thumb Taq Yes No Yes < 5 kb 1 min / kb ＊＊＊＊＊ ＊＊ ＊＊＊＊

BioFACT™ Taq Yes No Yes < 5 kb 1 min / kb ＊＊＊＊ ＊＊ ＊＊＊＊

BioFACT™ H-Star Taq Yes No Yes < 1 kb 1 min / kb ＊＊ ＊＊＊＊＊ ＊＊
BioFACT™ A-Star Taq Yes No Yes < 5 kb 1 min / kb ＊＊＊＊ ＊＊＊ ＊＊＊＊

BioFACT™ Lamp Taq Yes Yes w Yes < 40 kb 1 min / kb ＊＊＊＊＊ ＊＊＊ ＊＊＊
(λ DNA기준)

BioFACT™ A-Star Lamp Taq Yes Yes w Yes < 40 kb 1 min / kb ＊＊＊＊ ＊＊＊ ＊＊＊＊
(λ DNA기준)
BioFACT™ Pfu No Yes No < 3 kb 2 min / kb ＊＊＊ ＊＊ ＊＊

BioFACT™ Lamp Pfu No Yes No < 20 kb 0.5 min / kb ＊ ＊ ＊
BioFACT™ 2X Multi-Star PCR Yes No Yes < 1 kb 1 min / kb ＊＊ ＊＊＊＊＊ ＊＊

BioFACT™ RT Yes

BioFACT™ OneStep RT-PCR Yes No Yes < 1 kb 1 min / kb ＊＊ ＊＊＊＊＊ ＊＊ Yes
BioFACT™ Bst No No Yes

- Yes : Weak 3'→ 5' exonuclease ( delity) activity
W
- Yield : PCR ampli cation e ciency, amounts of end-product after PCR ampli cation
- Extension Time : Ampli cation length and extension time are di erent depending on the speci c characteristics of each PCR enzyme
- Speci city : Target speci city, ampli cation level for target product without non-speci c bands
- Sensitivity : Sensitivity for template DNA concentration, PCR ampli cation level according to the template DNA concentration

34 BIOFACT CATALOGUE