Page 31 - 2022-23 BIOFACT Catalogue

DNA / RNA Amplification Description

Efficiency Test

BIOFACT PCR Enzyme Quality Control System

Comparison Test of LOD
<Enzyme LOD test> <Template LOD test>
ApoE (0.9 kb) APEX (1 kb)
M A B C D E M M A B C D E F M M A B C D E F M

BIOFACT BIOFACT

Company A
Company A
Company B
Company B
Company C

Company C
Company D
Target : Human genomic DNA 10 ng PCR Condition : 60 °C, 35 cycles Target : Human genomic DNA PCR Condition : 60 °C, 35 cycles
M : 1kb DNA Ladder M : 1kb DNA Ladder
A : 2.5U B : 1.25U C : 0.625U D : 0.313U E : 0.156U A : 50 ng B : 10 ng C : 2 ng D : 0.4 ng E : 0.08 ng F : 0.016 ng

Comparison Test between BIOFACT PCR Enzyme and Other Companies' PCR Enzymes /
Comparison Test between BioFACT™ Taq and BioFACT™ A-Star Taq

BIOFACT Company A Company B Company C Company D
M A B C D E M A B C D E M A B C D E M A B C D E M A B C D E M
Target : Human genomic DNA 50 ng
Gene : β-Globin
PCR Condition : 60 °C, 35 cycles
M : 1kb DNA Ladder
A : 0.5 kb B : 1 kb
C : 2 kb D : 3.6 kb
E : 5 kb
NF-κB (1.2 kb) TNFR1
Taq A-Star Taq Taq A-Star Taq
M A B C D E F M A B C D E F M A B C D E F M A B C D E F
Target : Human genomic DNA
PCR Condition : 60℃, 35 cycles
M : 1kb DNA Ladder
A : 50 ng B : 10 ng C : 2 ng
D : 0.4 ng E : 0.08 ng F : 0.016 ng
BIOFACT Company A Company B Company C

M A B C D E F G M M A B C D E F G M M A B C D E F G M M A B C D E F G M

Template : Lambda DNA
PCR Condition : AT 68℃, 30 Cycles
M : 1kb DNA Ladder
A : 1 kb B : 3.6 kb C : 5 kb D : 10 kb
E : 14 kb F : 17 kb G : 22 kb

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BIOFACT CATALOGUE