Page 30 - 2022-23 BIOFACT Catalogue

Stability Test

BIOFACT PCR Enzyme Quality Control System

Stability Test on Various Temperature Ranges
<BioFACT™ H-Star Taq> <BioFACT™ Real-Time PCR Enzyme>
A B C D E F A B C D E F
-20℃ -20℃ -20℃ -20℃
72℃ 15 min 95℃ 15 min 72℃ 15 min 95℃ 15 min
72℃ 30 min 95℃ 30 min 72℃ 30 min 95℃ 30 min
72℃ 1 hr 95℃ 1 hr 72℃ 1 hr 95℃ 1 hr
72℃ 2 hr 95℃ 2 hr 72℃ 2 hr 95℃ 2 hr
72℃ 4 hr 95℃ 4 hr 72℃ 4 hr 95℃ 4 hr
A : 50 ng 50 ng
72℃ 8 hr 72℃ 8 hr
B : 10 ng 10 ng
C : 2 ng 2 ng
72℃ 12 hr 72℃ 12 hr
D : 0.4 ng 0.8 ng
E : 0.08 ng
72℃ 24 hr 72℃ 24 hr
F : 0.016 ng
Stability Test on Various Periods
<BioFACT™ Taq> <BioFACT™ F-Star Taq> <BioFACT™ Multi-Star PCR Enzyme>

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20℃ 4℃ 20℃ 4℃ 20℃ 4℃

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A B C D A B C D A B C D A B C D A B C D A B C D
1 week 1 week
1 week
4 week 4 week
10 week 10 week
16 week 16 week 4 week
30 week 30 week
56 week 56 week
10 week
80 week 80 week
p53 primer p53 primer
A : 2.5 U B : 1.25 U C : 0.62 U D : 0.31 U A : 2.5 U B : 1.25 U C : 0.62 U D : 0.31 U
<BioFACT™ Pfu> <BioFACT™ RTase> 16 week

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20℃ 4℃ 20℃ 4℃
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A B C D A B C D A B C D A B C D
1 week 1 week 30 week
4 week 4 week
10 week 10 week
56 week
16 week 16 week
30 week 30 week
56 week 56 week 80 week
80 week 80 week
GLO primer Human Total RNA 10 ng (Oligo dT(18)), β-Actin Rice primer
A : 2.5 U B : 1.25 U C : 0.62 U D : 0.31 U A : cDNA 5 0 B : cDNA 5 -1 C : cDNA 5 -2 D : cDNA 5 3 A : 2.5 U B : 1.25 U C : 0.62 U D : 0.31 U
• Long-term test : -20℃ (Expiration date 2 Years and 3 months, 821.25days)
• Acceleration test : 4℃ *TAA(Accelerated aging temp. / Desired Temp.) : 4℃ *TRT(Ambient Temp. / Preservation Temp.) : -20℃ *AAF(AAF = Q10 [(TAA-TRT)/10] ) : 5.27
*AAT : 821.25/5.27 = 155.83 days = 22.27 weeks (Accelerated aging conditions for expiration date, 2 years and 3 months)
30 BIOFACT CATALOGUE