Page 40 - 2022-23 BIOFACT Catalogue

LyoFACT™ PCR Pre-Mix

Lyophilized Typed PCR Pre-Mix

LyoFACT™ PCR Pre-Mix is designed to increase storage stability by freeze-drying solution-typed PCR Pre-Mix. Its ready to use format
can be stored at room temperature for long-term storage while remaining structurally and chemically stable as the moisture of it is
decreased to less than 1%, unlike the wet dry formulation. In addition, it is fully reconstituted after addition of water (rehydration).

Feature
• Source : Thermus aquaticus • Hot Start activity : Taq (No), Multi-Star (Yes(chemical-mediated))
6
• 5' → 3' exonuclease activity : Yes • 12-13 bp error / 10 bp
• 3' → 5' exonuclease activity (Fidelity) : No • A-tailing : Yes
• Amplification size : Taq < 5kb, Multi-Star <1kb • Amplification of more than one target in a single reaction (Multi-Star)

Application
- Taq - Multi-Star
• Routine PCR • Multiplex PCR ( ~ 13 bands in a parallel)
• PCR screening • Allele specific PCR ( ~ 21 bands in a parallel)
• TA cloning • Methylation specific PCR
• Colony PCR • SNP detection
• General sequencing
• TA cloning
• SSR / MS
• UDG system

QC DATA
- Stability test on various periods (Changes in cake shape)
기간별 안정성 테스트 [제조사별 Cake 형태 변화]
BIOFACT Company A Company B
타사 (B)
타사 (A)
BIOFACT

0 week

After
20 weeks

* 보관 온도 : 상온 * Cake 형태가 보관 후에 변형되면 제품의 성능이 저하될 우려가 있습니다.(타사 A : cake size 감소, 타사 B : 수분 생성)
* Storage temp. : 4 ℃
* There is a risk of performance degradation in case the shape of the cake is changed.
(Company A: cake size is decreased, Company B: moisture is created.)

40 BIOFACT CATALOGUE