Page 94 - 2022-23 BIOFACT Catalogue

BioFACT™

Lamp Pfu DNA Polymerase

Blunt-End Fragment Ampli cation of Less than 20 kb with High Accuracy

BioFACT™ Lamp Pfu DNA Polymerase is an upgraded version of existing Pfu DNA polymerase using mutagenesis to express the Pyro-
coccus furiosus DNA polymerase gene in E.coli. While retaining the features of BioFACT™ Pfu DNA Polymerase, the extension time is
signi cantly reduced to 30 seconds per 1kb and the ampli cation rate is much better than that of Pfu DNA Polymerase.
In addition, it enables the ampli cation of long PCR fragments of 20 kb (based on human gDNA). This enzyme also allows blunt
cloning without an additional A-tailing process using the All in One™ PCR Cloning Kit.

Feature
• Source : Pyrococcus furiosus • A-tailing : No
• 5' → 3' exonuclease activity : No • 4X faster processivity than Pfu DNA polymerase
• 3' → 5' exonuclease activity ( delity) : Yes • Error rate : 1-2 bp or less error/ 10 bp
6
• Ampli cation size : < 20 kb PCR

Application
• High delity PCR • Blunt end form cloning
• Site-directed mutagenesis • Long PCR (<20 kb)

QC DATA
- Comparison of activity by concentration of enzyme - Comparison of activity by concentration of template
between BioFACT™ Lamp Pfu DNA Polymerase and between BioFACT™ Lamp Pfu DNA Polymerase and
other Pfu DNA Polymerase other Pfu DNA Polymerase
BIOFACT Company A BIOFACT Company A
M A B C D E M M A B C D E M M A B C D E F M M A B C D E F M
APEX APEX
(1 kb) (1 kb)
NF-κ B NF-κ B
(1.2 kb) (1.2 kb)

SOX2 SOX2
(1 kb) (1 kb)
TNFR1 TNFR1
(0.7 kb) (0.7 kb)
p53 p53
(1.1 kb) (1.1 kb)

GLO GLO
(5 kb) (5 kb)
Target : Human genomic DNA 50 ng PCR Condition : 60°C, 35 cycles Target : Enzyme 1.25 unit PCR Condition : 60°C, 35 cycles
M : 1kb DNA Ladder M : 1kb DNA Ladder
A : 2.5 U B : 1.25 U C : 0.625 U D : 0.313 U E : 0.156 U A : 50 ng B : 10 ng C : 2 ng D : 0.4 ng E : 0.08 ng F : 0.016 ng

94 BIOFACT CATALOGUE