Page 90 - 2022-23 BIOFACT Catalogue

BioFACT™ A-Star

Lamp Taq DNA Polymerase

Antibody-Mediated Hot Start PCR Enzyme for Long Range PCR

BioFACT™ A-Star Lamp Taq DNA Polymerase is a blended enzyme of Taq DNA polymerase and Pfu DNA Polymerase processing proofread-
ing capability. It o ers the speci city and sensitivity of Hot Start polymerase through anti-Taq monoclonal antibody that inactivates the
enzyme until the rst denaturation step, avoiding extension of non-speci c annealed primers or primer-dimers.
BioFACT™ A-Star Lamp Taq DNA Polymerase is highly suitable for e cient ampli cation of di cult templates caused by high GC content
or complex structure and it is especially ideal for accurately amplifying long fragments with high yields and speci city.

Feature
• Source : Pyrococcus furiosus & Thermus aquaticus • Hot Start activity (Antibody-mediated Hot Start) : Yes
• 5'→ 3' exonuclease activity : Yes • Long range PCR Enzyme
• 3'→ 5' exonuclease activity ( delity) : Yes (weak) • High sensitivity & high speci city
• Ampli cation size : <40 kb, based on λDNA • Antibody mediated Hot Start PCR enzyme
• A-tailing : Yes • Facilitation of low copy DNA detection

Application
• Long range PCR (<40 kb, based on λDNA) • TA cloning
• Di cult PCR • Colony PCR
• High productivity PCR • Suitable for UDG system
• Inverse PCR

QC DATA
- Comparison of LOD according to various enzyme units - Comparison of LOD according to various template
between BioFACT™ A-Star Lamp Taq DNA Polymerase concentrations between BioFACT™ A-Star Lamp Taq
and the other company's PCR enzyme DNA Polymerase and the other company's PCR enzyme
BIOFACT Company A BIOFACT Company A
M A B C D E M M A B C D E M M A B C D E M M A B C D E M
APEX (1 Kb) APEX (1 Kb)

TNFR1 (722 bp)
TNFR1 (722 bp)
p53 (1.1 Kb)
GLO (1 Kb)
GLO (1 Kb)
GLO (5 Kb)
GLO (5 Kb)
Target : Human genomic DNA 50 ng PCR Condition : 60°C, 35 cycles Target : Human genomic DNA 50 ng PCR Condition : 60°C, 35 cycles
M : 1kb DNA Ladder M : 1kb DNA Ladder
A : 2.5 U B : 1.25 U C : 0.625 U D : 0.313 U E : 0.156 U A : 50 ng B : 10 ng C : 2 ng D : 0.4 ng E : 0.08 ng F : 0.016 ng

90 BIOFACT CATALOGUE