Page 217 - pathology_services_handbook_5th_edition_2018

IV PARASITOLOGY

4.1 Blood Film Malaria Parasite (BFMP)
4.1.1 Materials required :

a. Frosted slide MICROBIOLOGY
b. Slide jacket
c. Clean gauze
d. Alcohol (70%)
e. Clean cotton wool
f. Even, flat area to dry slides
g. Sterile Lancet (finger prick) or sterile needle/syringe 3 ml (syringe method)

4.1.2 Method :

a. Clean slides with gauze soaked in alcohol (70 %).Do not use cotton.
b. Dry slides using gauze.
A. Finger prick

i. Select third finger from the thumb (big toe can be used for infants). Never use
thumb, for either children or adults. Massage it to get better blood flow.
ii. Clean finger (or toe) with cotton wool soak in alcohol (70%). Use firm strokes to
remove dirt and oils from the ball of the finger.

iii. Dry the finger with clean cotton wool. Use firm strokes to stimulate blood
circulation.
iv. Puncture the ball of the finger with a sterile lancet using a quick rolling motion.

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