Page 218 - pathology_services_handbook_5th_edition_2018

HTAA, KUANTAN

v. Apply gentle pressure to the finger (or toe) and express the first drop of blood.
Wipe it away with dry cotton wool, make sure that no cotton strands remain
thatmight later be mixed with the blood.
vi. Work quickly and handling the slides by the edges, collect the blood.

vii. Allocate 2 cm from one end of the slide for labeling with sticker.
viii. Drop 1 big drop/3 small drops onto slide. This is the thick film.
ix. Space out 1 cm, collect a single drop about the middle of the slide. This is the
thinfilm. Wipe the remaining blood off the finger with cotton wool.
x. Place slide onto a flat surface.

xi. Take another slide to be use as a spreader, put it at an angle of 30-45°, and
touchthe single drop of blood. Allow the blood to run right along the edges.
xii. Firmly push the spreader forward along the slide, keeping it at an angle of 45°.
The edge of the spreader must remain in even contact with the surface of the
other slide while the blood is being collected.

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