Page 48 - Abstract book - TJSSF-2020

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”

Growth Inhibitory Activities of Crude Extracts Obtained from Local
Plants against Escherichia coli

Thanachot Boonmak , Satawat Kerdtin , Wiwat Upakarneekit
1
1
1
Advisors: Nutthaya Konklong , Supa Singtookreaw
1
1

Watjuntrawas (Sukprasarnrat) School
1

Abstract
This study was a test to compare the activity against Escherichia coli causing diarrhea or food poisoning
by using coarse extracts in ethanol from 5 local plants in the ratio of 1: 10 grams per volume at room
temperature. From the paper disc diffusion inhibition test, it was found that the crude extract from
Wildbetal Leafbush (piper sarmentosum) had the best inhibitory effect, followed by the lemongrass
(Cymbopogon citratus) with the clear diameter of the inhibitory zone. (Inhibition zone) equals 18.69 ±
1.2 and 11.22 ± 2.59 mm, respectively. And no inhibitory effect was found in Water hyacinth
(Eichhornia crassipes), Guava (Psidium guajava) and Kariyat (Andrographis paniculata) when
considering the efficacy of crude extract from Wildbetal Leafbush to inhibit the growth of Escherichia
coli bacteria. It was found that the efficiency of the extract increased with the clear zone, the diameter
of the clear zone increased from 10.89 mm to 18.69 mm. When increasing the concentration of 80% to
100%. In addition, the team developed a program to control automatic spraying devices from the
Kidbright board to stop the growth of Escherichia coli bacteria in the bathroom by creating a bathroom
model with the installation of a spraying device. And inquire about the opinions of users in 3 areas
which are physical, usage and safety Found that the satisfaction was at a high level with an average of
4.34

Keywords: Crude extract in the ethanol layer, Escherichia coli, paper disc diffusion, Kidbright

36