Page 50 - Abstract book - TJSSF-2020

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”

Biomaterials based on Alginate and Polyvinylalcohol for Wound Healing
in the Laboratory

Baibua Borkird , Montraluk Prommanee
1
1
Advisors: Adinan Jehsu , Jirat Meesane , Jutakan Thonglam , Yadanar Mya Moe
2
1
2
2

PSU Wittayanusorn School
1
Prince of Songkla University
2

Abstract
In present day, synthetic biomedical material is used for tissue engineering, especially in the wound
healing. The commercial membrane becomes an alternative way, and it has a high ability for new tissue
formation. However, this membrane has high cost for patient implantation. In this study choose alginate
(complex carbohydrate is extracted from brown seaweed) that is common and cheap. The research of
Burapha University and Silpakorn University, Applications of Polymers from Marine Resource, shows
that alginate is able to inhibit inflammation and bacteriostatic action. Polyvinyl alcohol usually is used
for welding substance to be dense. Alginate and Polyvinyl alcohol (PVA) are polymers that can
fabricate into membrane form. Moreover, the biofunctionality of these polymers support for cell
migration and proliferation. In this study, the 5% by mass per unit volume of alginate and PVA is
combined at different ratio including 100:0, 70:30, 50:50, 30:70 and 0:100 3 ml per piece in square
mold that is examined 15 pieces each ratio. The freeze thawing method at -80 degree celsius is selected
for membrane fabrication and Freeze dry is conserved qualification of membrane. Calcium Chloride
was used for soaking the membrane in the last process to improve the mechanical property of the
membrane. This productive process is tested for following cell growth. Photomicroghaphy can be
recorded from using confocal microscope. Finally, overall are utilized to prepare the most effective for
cell growth that is a principal part of wound healing. The 70:30 of Alginate/PVA showed the good
performance for wound healing application. The concentration of solution results in forming. The first
experiment, which is mixed alginate solution with a concentration of 1%w/v and the PVA solution is
concentrated. The mass to volume ratio, which defines the process leading to a freeze thow result is a
mixture of such a change is a gel but has not been used enough in a gel adhesive.

Keywords: Fibroblast, Polyvinylalcohol, Alginate

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