Page 404 - Clinical Hematology_ Theory _ Procedures ( PDFDrive )

388 PART 6 ■ Neoplastic Disorders

TABLE 21.6 Laboratory Information

Morphology of blood cells % blasts, % dysplastic cells

Flow cytometry To con rm myeloid lineage: CD33+, CD13+, myeloperoxidase (MPO) +

Cytogenetics Karyotypic subtypes

Molecular mutations FLT 3-analysis required for all cases of suspected AML, NPM1, CEBPA, RUNX1, BCR-ABL 1, KIT,

(selected cases) other prognostic factors.

k m m x k , , k m , x m , AML

m m q z m ALL, AML.

.

P m - k AML m NOTE: This is a good time to complete Review Questions

k , NPM1 m , related to the preceding content.

F L-3, I D, DNM 3A m -

.

Characteristics of AML Subgroups

MicroRNAs AML z m mm -

MicroRNA x , m - m m -

m , m . T q m

AML. M RNA - - - f k . T M

RNA m m (F . . ). T

z m m mRNA q M M -

- m .

. M m m m

R , m RNA k m . M m -

m m k m . T M

m m , x m , erythroleukemias m -

m m . M RNA m . A m m ,

TABLE 21.7 Characteristics of Dysplasia

Cytoplasmic Morphology Examples

Cellular Lineage Nuclear Morphology Examples of Features of Features

Erythroid Unequal binucleation Ring sideroblasts

Multinucleation Coarse stippling

Internuclear bridging Incomplete hemoglobinization

Karyorrhexis/pyknosis Vacuoles

Nuclear budding/lobulation

Granulocytic Giant cells Nuclear/cytoplasm asynchrony

Hypersegmentation Hypogranularity

Coarsely condensed chromatin with long strand Pseudo Chédiak-Higashi granulation

connections between lobes Auer bodies

Hyposegmentation (pseudo Peger-Huët) Vacuoles

Megakaryocytic Micromegakaryocytes Giant platelets

Hypolobulation Hypogranular

CD34+