Page 9 - Ranger SPM 2022 - Science

Science SPM Chapter 1 Safety Measures in the Laboratory
Managing Biological Waste Substances
1. Biological waste substances are biological waste substances that can cause harm
or biological hazards.

2. The procedures of managing biological waste substances in the laboratory must
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be in accordance with Standard Operating Procedure (SOP).
3. Biological wastes are separated according to categories. Figure 1.4 is a flow chart
of the Standard Operating Procedure for biological waste disposal.

Form 4

Category A Category B Category C Category D
Sharp equipment Non-sharp solids Carcasses, organs Liquid
(syringe, needle, (gloves, tissue, culture (lab animals and (blood, serum)
scalpel blade) medium) experimental
animals)

Placed in special Packaged in a Wrapped in Decontaminated
containers biohazard plastic bag absorbent material by autoclave
(sharps waste bins) and decontaminated (tissue paper) and
by autoclaving at a then packaged
temperature of 121°C, neatly in a
a pressure of 15 psi biohazard plastic
for 20 minutes bag

After autoclaving, tie Stored temporarily Disposed of
up the plastic bag and in a freezer directly into
label it sewage system
through the sink
or toilet

Stored temporarily in a special storage location such as a
biohazard bin under safe, controlled condition and cooled.

Centralised disposal is carried out

Figure 1.4 Flow chart of Standard Operating Procedure for biological waste disposal

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