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Biology SPM  Chapter 2 Leaf Structure and Function
                      Table 2.2 Comparison between light-dependent reactions and light-independent reactions
                                                    Similarities

                   •  Both occurs in chloroplast.
                   •  Both are catalysed by enzymes.
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                                                    Differences
                           Aspects            Light-dependent reactions  Light-independent reactions
                   Reaction site            Thylakoid                 Stroma

                   Substance required       Water                     Carbon dioxide
                   Product of reactions     Oxygen and water          Glucose
                   Photolysis of water      Present                   Absent
                   Reduction of carbon dioxide  Absent                Present
                   ATP and NADPH            Produce ATP and NADPH     Utilise ATP and NADPH
                                                                                                      Form 5
                  Environmental  Factors  that  Affect the  Rate  of Photosynthesis

                                                      •  Light energy is needed for light photolysis and to
                                                       activate chlorophyll in light-dependent reactions.
                                                      •  As light intensity increases, photosynthesis rate
                                                       increases until it reaches the saturation level at
                                                       point P (Graph I).
                        Photosynthesis rate   (cm/ min)  P Graph I  at 30ºC  2  •  Limiting factors such as carbon dioxide and
                              Graph II
                                                      •  After point P, increase in light intensity no longer
                                          0.13% CO
                                                2
                                                       causes increase in photosynthesis rate.
                                          0.03% CO
                                          at 30ºC
                                                       temperature had limited the increase of
                                                       photosynthesis rate.
                                                       increasing concentration of carbon dioxide or
                                 Light intensity (Lux)  •  Photosynthesis rate can be increased by
                                                       temperature (Graph II).
                                                      •  When light intensity is too high, photosynthesis
                                                       rate slows down because chlorophyll pigments are
                                                       damaged by ultraviolet rays.
                                                      •  Carbon dioxide is needed to synthesise glucose in
                          30ºC at high light          •  As the carbon dioxide increases, photosynthesis
                                                       light-independent reactions.
                          intensity
                       Photosynthesis rate   (cm/ min)  30ºC at high  •  After the saturation point, increase in concentration
                                                       rate increases until it reaches saturation point.
                                                       of carbon dioxide no longer increases the rate of
                                                       photosynthesis.
                                    light intensity
                                                      •  Limiting factors such as light intensity and
                                                       temperature limit the increase in photosynthesis
                         Concentration of carbon dioxide (%)
                                                       rate after the saturation point.




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