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Sains Tingkatan 5 Bab 1
6. Simpan piring Petri D di dalam peti sejuk yang ditetapkan pada suhu 5 °C.
Store Petri dish D in a refrigerator set at 5°C. 1
7. Perhatikan dan catatkan bilangan koloni bakteria selepas dua hari.
Observe and record the number of bacterial colonies after two days. BAB
Kaedah 1. Empat piring Petri steril yang mengandungi agar-agar nutrien steril dilabelkan sebagai A, B,
Method
C dan D.
EKSPERIMEN WAJIB
Four sterile Petri dishes containing sterile nutrient agar were labelled as A, B, C and D.
2. 1 cm³ kultur bakteria diletakkan ke atas permukaan agar-agar di dalam setiap piring Petri itu.
1 cm³ of bacterial cultures were placed onto the surface of the agar in each Petri dish.
3. Piring-piring Petri itu ditutup dengan penutup dan dilekatkan dengan pita selofan.
The Petri dishes were covered with lids and the lids were sealed using cellophane tape.
4. Piring Petri A dan B masing-masing disimpan di dalam ketuhar yang ditetapkan pada suhu
60°C dan 37°C.
Petri dishes A and B were stored in ovens set at 60°C and 37°C respectively.
5. Piring Petri C disimpan di dalam makmal pada suhu bilik, kira-kira 30°C.
Petri dish C was stored in the laboratory at room temperature, approximately 30°C.
6. Piring Petri D disimpan di dalam peti sejuk yang ditetapkan pada suhu 5°C.
Petri dish D was stored in a refrigerator set at 5°C.
7. Bilangan koloni bakteria diperhatikan dan dicatatkan selepas dua hari.
The number of bacterial colonies was observed and recorded after two days.
K1P5
Pemerhatian
Observation Piring Petri Suhu (°C) Bilangan koloni bakteria
Petri dish Temperature (°C) Number of bacterial colonies
K3
PI A 60 Tiada / None
1
2
3 B 37 Sangat banyak / Vast
S
C 30 Banyak / Many
D 5 Tiada / None
K3P1 K3P2 K3P3
Perbincangan 1. Berikan satu inferens tentang bilangan koloni bakteria pada suhu 60°C dan 5°C.
Discussion Give an inference on the number of bacterial colonies at temperatures 60°C and 5°C.
K4 Suhu-suhu itu tidak sesuai untuk pertumbuhan bakteria.
PI not suitable
1 The temperatures are for the growth of bacteria. K4P1
2
3 2. Berdasarkan pemerhatian, tandakan (✓) suhu optimum untuk bakteria bertumbuh.
S Based on the observation, mark (✓) the optimum temperature for the bacteria to grow.
5°C ✓ 37°C 60°C
Aktiviti Interaktif
GURU Kesan Suhu terhadap Pertumbuhan Bakteria
GURU
Effect of Temperature on the Growth of Bacteria
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