Page 20 - REC :: M.Tech. BioTech Curriculum and Syllabus - R2019

BY19311 ADVANCED MOLECULAR BIOLOGY AND GENETIC Category L T P C
ENGINEERINGLABORATORY
0 0 6 3

Objectives:
 To learn and understand the principles behind the cloning and expression of agene
 To perform nucleic acidassays
 To study the recombinant proteinexpression

List of Experiments
1 Isolation of Genomic DNA and Plasmid DNA from bacteria
2 Restriction Digestion and ligation of the plasmid vector
3 Transformation to E.coli
4 Polymerase chain reaction.
5 Colony PCR
6 Gel elution of DNA fragments.
7 Optimisation of inducer time and concentration for recombinant protein expression.
8 Western blotting analysis
9 Extraction of RNA
10 cDNA preparation from RNA
11 Site directed mutagenesis
12 Southern blotting – Non radioactive
Total Contact Hours : 90

Course Outcomes:
 Understand the basic principles of molecular biotechnology andassays
Obtain practical knowledge in analysing nucleic acid molecules both quantitatively and

qualitatively
 Gain knowledge in concept of geneticengineering
Acquire ability to use PCR techniques, to create site directed mutagenesis and detect disease-

causingmicrobes.
 Learn various techniques to make transgenic plants and transgenicanimals

Web links for virtual lab (if any)
Green M.R and Sambrook J Molecular cloning -A laboratory manual 4th Edition, Cold spring habor
1
laboratory press, USA,2012.
Zyskind J.W and Bernestin S.I Recombinant DNA laboratory manual Revised edition, Academic press ,
2
USA1992.

BY19312 BIOPROCESS AND DOWNSTREAM PROCESSING Category L T P C
LABORATORY
0 0 6 3

Objectives:
This course aims to provide hands on training in Bioprocess and Downstream Processing Lab by performing

enzyme kinetics, immobilization techniques and medium optimizationmethods.
To make the students understand the different methods involved in isolation, extraction of components,

purification and preservation ofproducts.

List of Experiments
1 Enzyme kinetics, inhibition, factors affecting reaction pH,temp.