Page 24 - REC :: M.Tech. BioTech Curriculum and Syllabus - R2019

UNIT-I CELLULARMETABOLISM 9
Transport Processes – Fueling reactions – Glycolysis, fermentative pathways – TCA cycle and oxidative
phosphorylation, anaplerotic pathways – Catabolism of fats, organic acids, and aminoacids - Biosynthesis of
aminoacids, nucleic acids, and fatty acids – Polymerization – Growth energetics.
UNIT-II REGULATION MANIPULATION AND SYNTHESIS OF METABOLIC PATHWAYS 9
Regulation of enzyme activity – Regulation of enzyme concentration – Regulation of metabolic networks –
Regulation at the whole cell level – Metabolic pathway manipulations – Enhancement of Product yield and
productivity – Extension of substrate range, product spectrum and novel products (Antibiotics, Polyketides,
Vitamins) – Improvement of cellular properties – Metabolic pathway synthesis algorithm – Lysine biosynthesis.
UNIT-III ANALYSIS AND METHODS FOR THEMETABOLIC FLUX 9
Metabolic flux map – Fluxes through the catabolic pathways in microbes – Metabolic flux analysis for
determined, overdetermined and underdetermined systems – Sensitivity analysis – Direct flux determination
from fractional label enrichment – Applications involving complete enumeration of metabolite isotopomers –
Carbon metabolite balances.
UNIT-IV APPLICATION OF METABOLICFLUXANALYSIS 9
Amino acid production – Biochemistry and regulation – Metabolic flux analysis of lysine biosynthetic network
and specific deletion mutants – Metabolic fluxes in mammalian cell cultures – Intracellular fluxes, validation of
flux estimates by C labeling studies – Design of cell culturemedia.
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UNIT-V ANALYSIS OF METABOLIC CONTROL AND STRUCTURE OF METABOLIC 9
NETWORKS
Fundamentals of metabolic control analysis (MCA) – Determination of flux control coefficients – MCA of
linear and branched pathways – Theory of large deviations – Branched and unbranched networks – Control of
flux distribution at a single branch point – Grouping reactions.
Total Contact Hours : 45

Course Outcomes:
 Understand the current advances in systemsbiology
 Gain insights into the field of metabolicengineering
 Biosynthesis of primary & secondary metabolites,bioconversions.

Text Book(s):
Stephanopoulos, G.N., Aristidou, A.A. and Nielsen.J., “Metabolic Engineering - Principles and
1
Methodologies”, Elsevier Science,2001.
Cortossa, S., Aon, M.A., Iglesias, A.A. and Lloyd.D., “An Introduction to Metabolic and Cellular
2
Engineering”, World Scientific Publishing Co,2002.

Reference Books(s) / Web links:
Scheper, T., “Metabolic Engineering – Advances inBiochemical Engineering Biotechnology”,
1
Springer,2001.
Curran, C.P., “Metabolic Processes and Energy Transfers - An Anthology of Current Thought”, The
2
Rosen Publishing group, Inc.,2006.
Nielsen, J., Villadsen, J. and Liden, G., “Bioreaction Engineering Principles”, 2 Edition, Kluwer Acadmic /
nd
3
Plenum publishers,2003.

BY19P14 ONCOGENETICS Category L T P C
3 0 0 3

Objectives:
 To enable the students to know cell cycle dysregulation in cancer and various stages of carcinogenesis.
 To understand the molecular basis of cancer and propose new treatment options for cancerpatients

UNIT-I PRINCIPLES OFCANCERBIOLOGY 9
Cancer: Definition, causes, properties, classification, clonal nature – Cell Cycle: Regulation of cell cycle, cell