Page 19 - REC :: M.Tech. BioTech Curriculum and Syllabus - R2019

Sharma, Brij Kishore, “Introduction to the Constitution of India”, Prentice Hall of India, New Delhi, 7 edition,
th
1
2015.
Subhash Kashyap, “Our Constitution : An Introduction to India’s Constitution and Constitutional Law”, National
2
Book Trust India, 1994.
th
3 Mahendra Prasad Singh and Himanshu Roy, “Indian Political System”, Pearson India, 4 edition, 2017.

BY19211 IMMUNOTECHNOLOGY LABORATORY Category L T P C
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Objectives:
To teach advanced techniques and skills required in diagnosis, treatment and research in

Immunotechnology.
 To acquire knowledge concerning the principles and applications of immunoassay procedure.

List of Experiments
1 Selection and handling of animals for immunologicalexperiments
2 Collection of Blood, Serum andPlasma
3 Blood smear identification of leucocytes by Giemsastain.
4 Isolation and identification oflymphocytes.
5 Preparation microbial antigen frompathogens
6 Administration of antigen and rising of antiserum in testanimal
7 Purification of IgG by Precipitationtechnique.
8 Slide and Tube agglutinationreaction
9 Qualitative analysis of antigen or antibody byELISA
10 Characterisation of antigens byImmunoblotting.
11 Chromatographic immunoassay(CEA)
12 ImmunofluorescenceTechnique
Total Contact Hours : 90

Course Outcomes:
 Isolate, Identify and characterization of various immunecells.
 Have knowledge on antigen preparation and immunizationtechniques.
Learn techniques like developing diagnostic tests, purification of antibody, Antigen –Antibody engineering etc

for industrialapplications
 Access health problems with an immunologicalbackground.
 Develop approaches for immuneintervention.

Web links for virtual lab (if any)
nd
Antibodies: A Laboratory Manual, Ed Harlow, David P Lane, Cold Spring Harbor Laboratory Press, 2
1
Edition, 1998
Current protocols in immunology / editorial board John E. Coligan .et al,. 2003, New York : Wiley
2
Interscience,2003
3 Ashim K. Chakravarthy, Immunology, TataMcGraw-Hill,1998.