Page 17 - REC :: M.Tech. BioTech Curriculum and Syllabus - R2019

BY19205 ADVANCED GENOMICSAND PROTEOMICS Category L T P C
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Objectives:
 Be familiar to the basic biology of modern genomics and the experimental tools that can be used to measureit.
 Be able to discuss the key technological developments that enabled modern genomic and proteomic studies.
 Understand principles and technologies for generating genomic information for biotechnological applications.

UNIT-I INTRODUCTION TO GENOME ANDGENESTRUCTURE 9
Introduction: Genome, Genomics, Omics and importance, History of genome projects, Organization and structure of
genomes in prokaryotes, eukaryotes, and organelles (chloroplast, mitochondrion); Genome mapping methods (Genetic
Mapping –i)Cross breeding and pedigree analysis, ii)DNA markers – RFLPs, SSLPs, SNPs and Physical Mapping –
Restriction mapping, Fluorescent in situ hybridization, Radiation hybrid mapping and Sequence tagged site mapping);
Advances in gene finding and functionalprediction
UNIT-II LARGE SCALE GENOMICS/ FUNCTIONALGENOMICSANALYSES 9
Genome projects: The Human genome project, HapMap Project, The 1000 genome project, and The ENCODE
Project. Structural genomics: Assembly of a contiguous DNA sequence- shotgun method, clone contig method,
and whole –genome shotgun sequencing ,Genome-wide association (GWA) analysis; Comparative Genomic
Hybridization (CGH); Massively parallel Signature Sequencing (MPSS); Whole genome shot-gun sequencing
and its applications. Introduction of Next Generation Sequencing (NGS). Pharmacogenetics – High throughput
screening in genome for drug discovery-identification of gene targets, Pharmacogenetics and drugdevelopment
UNIT-III TRANSCRIPTOMICS 9
Gene expression analysis by cDNA and oligonucleotide arrays; DNA microarray: understanding of microarray
(experimental analysis and data analysis), normalizing microarray data, detecting differential gene expression,
correlation of gene expression data to biological process and computational analysis tools (especially clustering
approaches).Methylome analysis using microarray; ChIP-on Chip analysis. Bioinformatic analysis of large-scale
microarray data for comparativetranscriptomics.
UNIT-IV SEPARATION AND PROCESSING OF PROTEINSFORPROTEOMICS 9
Over-view of strategies used for the identification and analysis of proteins; Protein extraction from biological
samples (Mammalian Tissues, Yeast, Bacteria, and Plant Tissues); 2-DE of proteins for proteome analysis;
Liquid chromatography separations in proteomics (Affinity, Ion Exchange, Reversed-phase, and size exclusion);
Enzymatic cleavage of proteins. Analysis of complex protein mixtures using Nano-liquid chromatography
(Nano-LC) coupled to Mass-spectrometry analysis.
UNIT-V MASS SPECTROMETRY ANDCOMPARATIVEPROTEOMICS 9
Common ionization methods for peptide/protein analysis; Introduction to Mass spectrometers; MALDI-TOF
and LC-MS analyses; Comparative proteomics based on global in-vitro and in-vivo abelling of proteins/peptides
followed by Mass-spectrometry. Analysis of post translational modification (PTM) of proteins; Characterization
of protein interactions using yeast two-hybrid system and Protein microarrays; Proteomics informatics and
analysis of protein functions.
Total Contact Hours : 45

Course Outcomes:
 Have basic knowledge about the methods used for genomics and proteomics.
 Apply functional genomics techniques in thelaboratory
 Familiar with how the methods are applied in real-life scientificresearch.
 know where to access the immense volumes of –omicsdata
The students will acquire in-depth knowledge on the methods and approaches in genomics and proteomics

areas which help them to carry out cutting edge academic and industrialresearch.

Text Book(s):
1 S.P. Hunt and F. J. Livesey, (2000) FunctionalGenomics
2 N. K. Spur, B. D. Young, and S. P. Bryant (1998) ICRF Handbook of Genome Analysis Volume 1 & 2
3 G. Gibson and S. V. Muse (2002) A primer of GenomeScience
4 R. J. Reece (2004) Analysis of Genes andGenomes
5 Rinaldis E. D. And Lahm A (2007)DNA Microarrays. Horizonbioscience.