Page 50 - B.Tech Biotech Curriculum and Syllabus R2017 - REC
P. 50
Department of BIOTECHNOLOGY, REC
To demonstrate about gas -liquid, vapour- liquid and solid- liquid and liquid–liquid equilibrium.
To classify and use the accurate engineering correlations of diffusion and mass transfer coefficients to
model a separation process.
To investigate a multi-stage equilibrium separation processes, simultaneous phase equilibrium and
mass balances in continuous separation processes (absorbers, strippers, and distillation columns) and
sizing continuous separation units.
To design and construction with operating principles of process economics of separating equipments
TEXT BOOKS
1. Treybal R.E. Mass Transfer Operations.3rd edition. McGraw-Hill, 1981.
2. Geankoplis C.J. Transport Processes and Unit Operations.3rd edition, Prentice Hallof India, 2002.
REFERENCE
1. Coulson and Richardson‘s Chemical Engineering. Vol. I & II, Asian Books Pvt. Ltd,
BT17503 MOLECULAR BIOLOGY L T P C
4 0 0 4
OBJECTIVES:
Familiarize students with the cell and molecular biology of both Prokaryotes and Eukaryotes. This
will be needed for any project work in modern biotechnology.
This course will emphasize the molecular mechanism of DNA replication, repair, transcription,
protein synthesis and gene regulation in various
UNIT I CHEMISTRY OF NUCLEIC ACIDS 10
Introduction to nucleic acids: Nucleic acids as genetic material, Structure and physicochemical properties of
elements in DNA and RNA, Biological significance of differences in DNA and RNA. Primary structure of
DNA: Chemical and structural qualities of 3‘, 5‘-Phosphodiester bond. Secondary Structure of DNA: Watson
& Crick model, Chargaff‘s rule, Conformational variants of double helical DNA, Hogsteen base pairing,
Reversible denaturation and hyperchromic effect.
UNIT II DNA REPLICATION & REPAIR 10
Overview of Central dogma.Organization of prokaryotic and eukaryotic chromosomes. DNA replication:
Meselson & Stahl experiment, bi–directional DNA replication, Okazaki fragments, Proteomics of DNA
replication, Fidelity of DNA replication, Inhibitors of DNA replication, Telomere replication in eukaryotes.
D-loop and rolling circle mode of replication.Mutagens, various types of repair mechanisms.
UNIT III TRANSCRIPTION 8
Structure and function of mRNA, rRNA and tRNA.Characteristics of promoter and enhancer sequences. RNA
synthesis: Initiation, elongation and termination of RNA synthesis, Proteins of RNA synthesis, Inhibitors of
transcription, Ribozymes, RNA processing: 5‘-Capping, Splicing-Alternative splicing, Poly ‗A‘ tail addition.
UNIT IV TRANSLATION 10
Introduction to Genetic code: Elucidation of genetic code, Codon degeneracy, Wobble hypothesis and its
importance, Prokaryotic and eukaryotic ribosomes. Steps in translation: Initiation, Elongation and termination
of protein synthesis. Inhibitors of protein synthesis.Post-translational modifications and its importance.
UNIT V REGULATION OF GENE EXPRESSION 7
Organization of genes in prokaryotic and eukaryotic chromosomes, Prokaryotic gene regulation –lac and trp
operon, Regulation of gene expression with reference to λ phage life cycle, Epigenetics.
TOTAL: 45 PERIODS
COURSE OUTCOMES:
By the end of this course, students should be able to:
Describe the basic structure and biochemistry of nucleic acids and proteins and discriminate between
Curriculum and Syllabus | B.Tech. Biotechnology | R2017 Page 50
