Page 53 - B.Tech Biotech Curriculum and Syllabus R2017 - REC

Department of BIOTECHNOLOGY, REC

REFERENCES
1. Bailey and Ollis, ― Biochemical Engineering Fundamentals‖, McGraw Hill (2nd Ed.), 1986.
2. Shuler and Kargi, ― Bioprocess Engineering ―, Prentice Hall, 1992.
3. Pauline Doran, Bioprocess Engineering Calculation, Blackwell Scientific Publications.
4. Peter F. Stanbury, Stephen J. Hall & A. Whitaker, Principles of Fermentation Technology, Science &
Technology Books.

BT17512 MOLECULAR BIOLOGY LABORATORY L T P C
0 0 4 2

OBJECTIVES:
 To learn and understand the principles behind the cloning and expression of a gene
 To perform nucleic acid assays
 To learn the recombinant protein expression

Experiments
1. Isolation of bacterial DNA
2. Isolation of genomic DNA from plant
3. Agarose gel electrophoresis (preparation of butters, reagents for AGE)
4. Isolation of total RNA
5. Competent cells preparation & transformation
6. Blue and white selection for recombinants
7. Phage titration
8. Isolation of genomic DNA from animal tissue
9. Check the purity of DNA by using UV spectrophotometer
10. Isolation of genomic DNA from yeast

TOTAL: 60 PERIODS

COURSE OUTCOMES:
Upon completion of the laboratory sessions, the students will be able to
 Clone and express a gene in a suitable host.
 Analyse nucleic acid molecules both quantitatively and qualitatively
 Gain knowledge in concept of genetic engineering
 create and express recombinant proteins.
 Produce genitically modified animals and plants.

REFERENCE
1. Sambrook, Joseph and David W. Russell ―The Condensed Protocols: From Molecular Cloning: A
Laboratory Manual‖ Cold Spring Harbor, 2006.

BT17513 BIOINFORMATICS LABORATORY L T P C
0 0 2 1

OBJECTIVES:
 To get qualified with programming knowledge.
 To enable the usage of bioinformatics tools.

LIST OF EXPERIMENTS

Basic UNIX commands

Types of Biological Databases and Using it.
Curriculum and Syllabus | B.Tech. Biotechnology | R2017 Page 53