Page 80 - B.Tech Biotech Curriculum and Syllabus R2017 - REC

Department of BIOTECHNOLOGY, REC

UNIT III FUNCTIONAL GENOMICS 9
Genome annotation, ORF and functional prediction,Gene finding,Substractive DNA library screening,
Differential display and Representational difference analysis, SAGE,TOGA, Introduction to DNA microarray.

UNIT IV TECHNIQUES IN PROTEOMICS 9
In-vitro and in vivo-labeling of proteins, One and two-dimensional gel electrophoresis, Detection of proteins
on SDS gels,Protein cleavage,Edman protein microsequencing, Massspectrometry- principles of MALDI-
TOF, Peptide mass fingerprinting.

UNIT V PROTEIN PROFILING 9
Large-scale protein profiling using proteomics, Post-translational modifications, Phosphoprotein and
glycoprotein analyses; Analysis of protein-protein interactions, Protein microarrays.

TOTAL: 45 PERIODS

COURSE OUTCOMES:
On completion of the course The students will
 gain a better understanding of the organization of genomes in multiple levels of taxa, and the
methodologies and approaches used for the study of structural and functional genomics.
 acquire knowledge on various genome mapping and sequencing methods, genomic markers,
microarray technology and methods for proteomics.
 Different methods used for genomics and proteomics .
 Apply functional genomics techniques in the laboratory
 The students will acquire in-depth knowledge on the methods and approaches in genomics and
proteomics areas which help them to carry out cutting edge academic and industrial research.

TEXTBOOKS
1. Suhai, Sandor ―Genomics and Proteomics: Functional and Computational Aspects‖. Springer, 2000
2. Pennington, S.R. and M.J. Dunn ―Proteomics: From Protein Sequence to Function‖. VivaBooks Pvt. Ltd.,
2002.
3. O‘Connor, C.D. and B.D.Hames. ― Proteomics‖. Scion Publishing, 2008.
4. Primrose, S.B. and Twyman. ―Principles of Genome Analysis and Genomics‖. 7th Edition,Blakwell
Publishing, 2006

REFERENCES
1. Cantor, Charles R. and Cassandra L. Smith. ―Genomics : The Science and Technology Behind the Human
Genome Project‖. John Wiley & Sons, 1999.
2. Liebler, R.C. ―Introduction to Proteomics‖. Humana Press, 2002.
3. Hunt, Stephen P. and Frederick J. Livesey. ―Functional Genomics‖. Oxford University Press,2000.
4. Conard, Edward. ―Genomics‖. Apple Academics, 2010

BT17E72 BIOENTREPRENEURSHIP AND IPR L T P C
3 0 0 3

OBJECTIVES:
 To enable the students
 To be aware of the risks and benefits of being an entrepreneur
 To apply the knowledge and become a successful entrepreneur and assist other entrepreneurs
UNIT-I: 9
Should You Become an Entrepreneur? What Skills Do Entrepreneurs Need? - Identify and Meet a Market
Need - Entrepreneurs in a Market Economy - Select a Type of Ownership

UNIT-II: 9
Develop a Business Plan

Curriculum and Syllabus | B.Tech. Biotechnology | R2017 Page 80