Page 76 - B.Tech Biotech Curriculum and Syllabus R2017 - REC

Department of BIOTECHNOLOGY, REC

UNIT V BIOCONJUGATE APPLICATIONS 9
Preparation of Hapten-carrier Immunogen conjugates - antibody modification and conjugation – immunotoxin
conjugation techniques – liposome conjugated and derivatives- Colloidal – gold-labeled proteins – different
methods of preparation and applications.

TOTAL : 45 PERIODS

COURSE OUTCOMES:
Upon completion of this course, the student would know about
 Modification and conjugation process of functional targets such as amino acids, proteins, sugars,
polysaccharides, and nucleic acids
 Chemistry of reactive functionalities with reference to modification and cross linking process
 Types, structures, properties and applications of bioconjugate reagents
 Enzymes and nucleic acid modification and conjugation
 Preparation of various bioconjugates and their applications

TEXT BOOKS:
1. Bioconjugate Techniques, G.T. Hermanson, Academic Press, 1999.

REFERENCE BOOKS:
th
1. Lehninger A.L., Nelson D.L. and Cox M.M. Principles of Biochemistry,6 edition, CBS publishers
and distributors, 2012.
th
2. LubertStryer W.H. Biochemistry, 5 Revised edition Freeman and company, New York, 2002.

BT17E67 FUNDAMENTALS OF NANOTECHNOLOGY L T P C
3 0 0 3

OBJECTIVES:
To learn about basis of nanomaterial science, preparation method, types and application.

UNIT I INTRODUCTION 8
Nanoscale Science and Technology- Implications for Physics, Chemistry, Biology and Engineering-
Classifications of nanostructured materials- nano particles- quantum dots, nanowires-ultra-thinfilms-
multilayered materials. Length Scales involved and effect on properties: Mechanical, Electronic, Optical,
Magnetic and Thermal properties. Introduction to properties and motivation for study (qualitative only).

UNIT II GENERAL METHODS OF PREPARATION 9
Milling, Colloidal routes, Self-assembly, Vapour phase deposition, MOCVD, Sputtering, Evaporation,
Molecular Beam Epitaxy, Atomic Layer Epitaxy, MOMBE.

UNIT III NANOMATERIALS 12
Nanoforms of Carbon - Buckminster fullerene- graphene and carbon nanotube, 92 Single wall carbon
Nanotubes (SWCNT) and Multi wall carbon nanotubes (MWCNT)- methods of synthesis(arc-growth, laser
ablation, CVD routes, Plasma CVD), structure-property Relationships applications- Nanometal oxides-ZnO,
TiO2,MgO, ZrO2, NiO, nanoalumina, CaO, AgTiO2, Ferrites, Nanoclays-functionalization and applications-
Quantum wires, Quantum dotspreparation, properties and applications

UNIT IV CHARACTERIZATION TECHNIQUES 9
X- ray diffraction technique, Scanning Electron Microscopy - environmental techniques, Transmission
Electron Microscopy including high-resolution imaging, Surface Analysis techniques- AFM, SPM, STM,
SNOM, ESCA, SIMS-Nanoindentation

Curriculum and Syllabus | B.Tech. Biotechnology | R2017 Page 76