Page 157 - 2022-23 BIOFACT Catalogue

DNA / RNA Preparation

- Comparison of gDNA isolation efficiency between HiGene™ Genomic DNA Prep Kit (Ver.2.0) [Column Type]
and other company's prep kits (column type)
<Microorganism - Gram- (E.coli)> OD 평균
E.coli Sample Conc. OD Total
Concentration
E.coli Sample Conc. (260/280) (260/280) Total 농도
[Average]
M BIOFACT Company A Company B Company C 1 1 58.7 ng/㎕
58.7 ng/µl
BIOFACT 2 56.5 ng/µl 2.00 6.0 ug
BIOFACT 2 56.5 ng/㎕ 2.00 6.0 ㎍
68.2 ng/µl
3 3 68.2 ng/㎕ OD 평균
1
20.0 ng/µl
E.coli 1 Sample 20.0 ng/㎕ Conc. (260/280) Total 농도
21.1 ng/µl
Company A A 사 2 2 21.1 ng/㎕ 2.28 2.28 2.1 ㎍ 2.1 ug
58.7 ng/µl
22.2 ng/µl
3 3 1 22.2 ng/㎕
BIOFACT 1 2 56.5 ng/µl 2.00 6.0 ug
57.4 ng/µl
1 57.4 ng/㎕
68.2 ng/µl
67.9 ng/µl
Company B B 사 2 2 3 67.9 ng/㎕ 2.10 2.10 6.2 ㎍ 6.2 ug
62.1 ng/µl
20.0 ng/µl
3 3 1 62.1 ng/㎕
49.0 ng/µl
21.1 ng/µl
A 사 1 1 2 49.0 ng/㎕ 2.28 2.1 ug
41.1 ng/µl
C 사 2 3 22.2 ng/µl 0.75 4.4 ug
Company C 2 41.1 ng/㎕ 0.75 OD 4.4 ㎍ 평균
Conc.
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading E.coli 3 Sample 44.2 ng/µl (260/280) Total 농도
57.4 ng/µl
3 1
44.2 ng/㎕
Elution volume : 100 ㎕ Cell number : 8 X 10 8 B 사 2 67.9 ng/µl 2.10 6.2 ug
1 3 58.7 ng/µl
62.1 ng/µl
BIOFACT 2 56.5 ng/µl 2.00 6.0 ug
1 49.0 ng/µl
C 사 3 2 68.2 ng/µl 0.75 4.4 ug
41.1 ng/µl
1 3 20.0 ng/µl
44.2 ng/µl
A 사 2 21.1 ng/µl 2.28 2.1 ug
OD
평균
Bacillus Sample 22.2 ng/µl (260/280) Total 농도
Conc.
3
1 57.4 ng/µl
<Microorganism - Gram+ (Bacillus)> B 사 1 2 29.6 ng/µl 2.10 6.2 ug
67.9 ng/µl
BIOFACT 2 3 28.9 ng/µl 2.15 2.9 ug
62.1 ng/µl
49.0 ng/µl
M BIOFACT Company A Company B M 3 1 29.6 ng/µl ODTotal 평균
26.8 ng/µl OD
Conc.
Concentration
4.4 ug
0.75
Bacillus Bacillus Sample Conc. 41.1 ng/µl (260/280) (260/280) Total 농도
1 2
C 사 Sample
[Average]
A 사 2 3 27.1 ng/µl 2.45 2.6 ug
44.2 ng/µl
1 3 1 29.6 ng/㎕
29.6 ng/µl
26.5 ng/µl
BIOFACT 1 2 28.9 ng/㎕ 2.15 2.9 ㎍ 2.9 ug
BIOFACT 2
29.5 ng/µl 2.15
28.9 ng/µl
29.6 ng/µl
28.8 ng/µl
B 사 3 2 3 29.6 ng/㎕ 2.45 2.8 ug
26.8 ng/µl
1 3 1 26.8 ng/㎕
27.9 ng/µl
Company A A 사 2 2 27.1 ng/㎕ 2.45 2.6 ㎍ 2.6 ug
27.1 ng/µl 2.45
26.5 ng/µl
3 3 26.5 ng/㎕ OD 평균
Bacillus 1 Sample 29.5 ng/µl (260/280) Total 농도
Conc.
1 29.5 ng/㎕
Company B B 사 2 2 28.8 ng/㎕ 2.45 2.8 ㎍ 2.8 ug
28.8 ng/µl 2.45
1
29.6 ng/µl
27.9 ng/µl
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading BIOFACT 3 3 27.9 ng/㎕ 2.15 2.9 ug
28.9 ng/µl
2
Elution volume : 100 ㎕ Cell number : 8 X 10 8 Blood Sample 29.6 ng/µl OD 평균
Conc.
3
1 1 26.8 ng/µl (260/280) Total 농도
37.1 ng/µl
A 사
BIOFACT 2 27.1 ng/µl 2.45 2.6 ug
1.92
3.9 ug
2 41.7 ng/µl
3 26.5 ng/µl
1 34.8 ng/µl
OD
A 사 1 29.5 ng/µl 1.79 3.4 ug
평균
Conc.
Blood Sample 34.4 ng/µl (260/280) Total 농도
2
28.8 ng/µl
2.8 ug
2.45
2
B 사
1 33.1 ng/µl
B 사 3 27.9 ng/µl 2.06 3.2 ug
32.4 ng/µl
37.1 ng/µl
BIOFACT 2 1 41.7 ng/µl 1.92 3.9 ug
<Whole Blood> C 사 1 2 2 1 21.8 ng/µl 0.62 2.1 ug
34.8 ng/µl
21.2 ng/µl
A 사 1.79 3.4 ug
2 34.4 ng/µl
BIOFACT Company A Company B Company C 1 33.1 ng/µl
M M B 사 2 32.4 ng/µl 2.06 Total 3.2 ug
OD
Whole Blood Sample Sample Conc. 21.8 ng/µl OD Concentration 평균
Blood
Conc.(260/280)
1
[Average]
0.62
C 사 (260/280) Total 농도
2.1 ug
21.2 ng/µl
1 2 37.1 ng/㎕
BIOFACT 1 37.1 ng/µl 1.92 3.9 ㎍
BIOFACT2 2 41.7 ng/㎕ 1.92 3.9 ug
41.7 ng/µl
1 34.8 ng/㎕
Company A 1 34.8 ng/µl 1.79 3.4 ㎍
A 사 2 34.4 ng/㎕ 1.79 3.4 ug
2 34.4 ng/µl
1 33.1 ng/㎕
Company B 1 33.1 ng/µl 2.06 3.2 ㎍
B 사 2 32.4 ng/㎕ 2.06 3.2 ug
2 32.4 ng/µl
1 21.8 ng/㎕
Company C 1 21.8 ng/µl 0.62 2.1 ㎍
C 사 2 21.2 ng/㎕ 0.62 2.1 ug
2 21.2 ng/µl
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading
Elution volume : 100 ㎕
157
BIOFACT CATALOGUE