Page 160 - 2022-23 BIOFACT Catalogue

QC DATA
- Comparison of extraction and PCR amplification efficiency _ Fungus

< Lysis using enzyme method > < Lysis using mechanical method>
M M M M
Genomic DNA
extraction
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕

M M

PCR
Amplification

M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading

- Comparison of extraction efficiency _ Microalgae filtered through the filter
1 2 3 M

sample ng /㎕ 260 / 280 260 / 230
1 Company A (For soil) 135.8 2.13 0.24
M : 1kb DNA Ladder BIOFACT
1. Company A (For soil) 2 (Lysis using enzyme method) 57.2 1.79 0.81
2. BIOFACT (Lysis using enzyme method) BIOFACT
3 394.5 1.93 2.17
3. BIOFACT (Lysis using mechanical method) (Lysis using mechanical method)

- Comparison of extraction and PCR amplification efficiency from various samples

Bacterium Fungus 1 Fungus 2 Fungus 3 Fungus 4
Gram(-) Gram(+) Yeast
M M 1 2 3 4 M 1 2 3 4 M 1 2 3 4 M 1 2 3 4

Gram(-) : E. coli, Gram(+) : S. aureus, Yeast : S. cerevisiae Fungus 1 : Cercophora sulphurella Fungus 2 : Alternaria [brassicae Fungus 3 : Botryotrichum piluliferum Fungus 4 : Aspergillus niger
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading 1 : Undiluted(5 0 ) 2 : 5 -1 3 : 5 -2 4 : 5 -3 target Gene : 18S rRNA ITS1/ITS4] M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading
Elution volume : 100 ㎕

160 BIOFACT CATALOGUE