Page 159 - 2022-23 BIOFACT Catalogue

Grinding Bead & Column Type DNA / RNA Preparation

HiGene™ Genomic DNA

Prep Kit For Microorganisms

Extraction of Microorganisms Genomic DNA without Expensive Fragmentation Equipment

HiGene™ Genomic DNA Prep Kit For Microorganisms is a column type kit that can extract high-quality genomic DNA from
gram-positive/negative bacterium, yeast and fungus. The kit includes a BioFACT™ grinding bead tube for e cient dissolution.
Additional enzymes such as high-concentration lyticase or lysozyme are unnecessary except proteinase K and genomic DNA can
be extracted easily and quickly using a general vortex mixer.

Feature
• Spin column type • Column binding capacity : 20 ㎍ DNA / Column
• Membrane : Glass micro ber

Application
• PCR (Real-Time PCR, Multiplex PCR, Long PCR and etc.) • Clone library construction
• Southern blotting analysis • Genotyping (SNP, SSR, AFLP, T-RFLP, etc.)

Workflow

- HiGene™ Genomic DNA Prep Kit For Microorganisms [GD101-100]

P1

Mechanical Column binding & Elute DNA Pure genomic DNA
lysis washing

Grinding time for each sample using BioFACT™ Grinding Comparison of extraction time
Bead Tube
Grinding Bead Time S. aureus E. coli S. cerevisiae
Sample Material Griding Time (min)
Tube 250
Gram-Negative Bacterium BioFACT™ 4 min. 200
e.g., Escherichia coli, Salmonella typhimurium P1 tube
150
Gram-Positive Bacterium BioFACT™ 12 min.
e.g., Staphylococcus aureus, Listeria monocytogenes P1 tube 100
Yeast BioFACT™ 50
e.g., Saccharomyces cerevisiae 12 min. 0
(If using 50 mg - 250 mg : 15 min. ~ 20 min.) P1 tube Competitor A Competitor B BioFACT
+ Lyticase + Lyticase
Filamentous fungus BioFACT™ 15 min. + Lysozyme + Lysozyme
e.g., Aspergillus spec., Alternaria brassicae P1 tube
The prep time of HiGene™ Genomic DNA Prep Kit for Microorganisms[Grinding Bead &
Column Type] was compared with competitor products by processing Staphylococcus
The prep time of HiGene™ Genomic DNA Prep Kit for Microorganisms[Grinding Bead & Column Type] was compared
aureus, Escherichia coli, and Saccharomyces cerevisiae.
with competitor products by processing Staphylococcus aureus, Escherichia coli, and Saccharomyces cerevisiae.
HiGene™ Genomic DNA Prep Kit shows shorter prep times in all three cases.
HiGene™ Genomic DNA Prep Kit shows shorter prep times in all three cases.
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BIOFACT CATALOGUE