Page 187 - 2022-23 BIOFACT Catalogue

DNA / RNA Preparation

- Guideline [Small Size Removal Condition] - Guideline [Dual Size Selective Condition]
PCR sample vol.: 50 ㎕ based PCR sample vol.: 50 ㎕ based
Approximate Size Cuto Ratio(v/v) Volume(㎕) Approximate First Selection Second Selection
Target size
1000 bp 0.6V 30.0 Ratio(v/v) Volume(㎕) Ratio(v/v) Volume(㎕)
800 bp 0.65V 32.5 700 bp 0.6V 30.0 0.8V 10
700 bp 0.7V 35.0 600 bp 0.65V 32.5 0.85V 10
600 bp 0.75V 37.5
500 bp 0.8V 40.0 550 bp 0.7V 35.0 0.9V 10
400 bp 0.9V 45.0 450 bp 0.75V 37.5 1.0V 12.5
300 bp 1.0V 50.0 350 bp 0.8V 40.0 1.3V 25
200 bp 1.3V 65.0
150 bp 1.5V 75.0 250 bp 0.9V 45.0 1.5V 30

QC DATA
- Comparison of purification - Comparison of extraction - Comparison of extraction
efficiency on various small fragments efficiency on various PCR product sizes efficiency on various DNA sizes

BIOFACT Company A BIOFACT Company A
0.65/0.85V 0.7/0.9V 0.75/1.0V 0.8/1.3V 0.9/1.5V 1.24/1.6V 1.1/1.45V 1.05/1.4V 1.0/1.3V 0.95/1.25V 0.9/1.5V 0.8/1.3V 0.75/1.0V 0.7/0.9V 0.65/0.85V 1.24/1.6V 1.1/1.45V 1.05/1.4V 1.0/1.3V 0.95/1.25V
M 0.5V 0.6V 0.65V 0.7V 0.75V 0.8V 0.9V 1.0V 1.3V 1.5V M M Input M M Input M

M : 1kb DNA Ladder 3% Agarose gel 10㎕ loading M : 1kb DNA Ladder 3% Agarose gel 10㎕ loading M : 1kb DNA Ladder 3% Agarose gel 10㎕ loading

Product Information
DaBead™ Size-Selective Cleanup Kit [NC701-100]

▶Contents
Size-Selective Reagent (Magnetic Bead)
Washing Bu er
Elution Bu er

Maker Cat.No. Product Size
BIOFACT NC701-100 DaBead™ Size-Selective Cleanup Kit 100 prep

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BIOFACT CATALOGUE