Page 184 - 2022-23 BIOFACT Catalogue

< Comparison of Sequencing analysis after PCR purification between column method and Magnetic Bead method >
<Column Type> <Bead Type>

* High quality sequencing data has been obtained through purification using DaBead™ PCR Purification Kit.

- Comparison of purification efficiency on various extraction types
< PCR purification of large number of samples using 96well press pipettor >
A B C D A B
M
M
M

M: 1kb DNA Ladder
A : Before purification B : Column C : FB Plate D : 96well press pipettor
* DNA recovery rate is similar with existing methods
M: 1kb DNA Ladder A : Before PCR purification B : After PCR purification 후

* High quality sequencing data has been obtained through purification using
DaBead™ PCR Purification Kit & DaBead™ 96well Magnetic Press Pipettor

- Comparison of cloning efficiency between column method Colony PCR results
and Magnetic Bead method
<Column Type>
PCR product is subjected to PCR puri cation under the same conditions using Col- NTC
umn Type and Magnetic Bead Type, and then check whether target insert DNA is
inserted through cloning and colony PCR after PCR puri cation through column
method and magnetic bead method.
<Column Type> <Magnetic Bead Type>
<Magnetic Bead Type>
NTC

* As a result of cloning into a product purified by the above two types, it was
confirmed that the insertion rate of insert DNA was similar between two types

184 BIOFACT CATALOGUE