Page 185 - 2022-23 BIOFACT Catalogue

DNA / RNA Preparation

- Removal of primer dimer during sequencing & cloning step
1. Accurate results can be obtained when sequencing step.
→ Through removal of primer dimer, it can avoid double peaks due to the dimer peak at the front part during sequencing step.
<Before Dimer Removal>

Dimer

<After Dimer Removal>

Dimer

2. Cloning e ciency of the target gene can be increased.
→ The probability of cloning of the dimer is higher than that of the target gene as cloning e ciency is higher for smaller sizes.
Cloning should be performed after removal of primer dimer.

Product Information
DaBead™ PCR Purification Kit [PP755-200]

▶Contents
MU MW1 Additive
MPW ME
MW1 Magnetic Bead
MW2 (empty bottle)

Reference
Kyeong-Ryeol Lee, et al. Increasing Monounsaturated Fatty Acid Contents in Hexaploid Camelina sativa Seed Oil by FAD2 Gene Knockout Using CRISPR-Cas9. Front.
Plant Sci., 29 June 2021.

Maker Cat.No. Product Size
PP755-200 DaBead™ PCR Puri cation Kit _ 1.5 ㎖ Magnetic stand 200 prep
PP855S-096 DaBead™ PCR Puri cation Kit _ 8well Pipettor 16 prep x 6 plate
BIOFACT PP855A-096 DaBead™ PCR Puri cation Kit _ APX-32P Auto prep machine 16 prep x 6 plate
PP955-096 DaBead™ PCR Puri cation Kit _ 96well Press Pipettor 96 prep x 1 Plate
PP455-096 DaBead™ PCR Puri cation Kit _ 48well Press Pipettor 48 prep x 2 plate
* Magnetic Bead can be purchased separately.
* Plasticwares : PP755-200 (Not included), Rest of them (Included)

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BIOFACT CATALOGUE