Page 194 - 2022-23 BIOFACT Catalogue

- Comparison of PCR purification efficiency using HiGene™ Gel & PCR Purification System between dimer removal and
high yield condition

M C 1 2 M
TNFR1 Gene

M : 1kb DNA Ladder
C : Control (before PCR Purification)
1 : HiGene™ Gel & PCR Purification System (Dimer removal condition)
2 : HiGene™ Gel & PCR Purification System (High yield condition)

• Dimer Removal Condition
1. Removal of primer dimer in PCR product regardless of size
2. Puri cation for 100 bp – 5 kb sized PCR product
• High Yield Condition
1. Puri cation for less than 100 bp sized PCR product
2. Puri cation for more than 5 kb DNA fragment
3. Gel extraction to obtain target band only

- Removal of primer dimer during sequencing & cloning step
1. Accurate results can be obtained when sequencing step.
→ Through removal of primer dimer, it can avoid double peaks due to the dimer peak at the front part during sequencing step.

<Before Dimer Removal>

Dimer

<After Dimer Removal>

Dimer

2. Cloning e ciency of the target gene can be increased.
→ The probability of cloning of the dimer is higher than that of the target gene as cloning e ciency is higher for smaller sizes.
Cloning should be performed after removal of primer dimer.

194 BIOFACT CATALOGUE