Page 198 - 2022-23 BIOFACT Catalogue
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BioFACT™ T4 DNA Ligase
Suitable Enzyme for All Common DNA Ligations
BioFACT™ T4 DNA ligase, widely used in both DNA repair and DNA replication, is isolated from a recombinant E.coli strain and
catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA
or RNA. It can join blunt-end and cohesive-end termini (Including sticky, ends for TA Cloning) as well as repair single stranded
nicks in duplex DNA, RNA, or DNA/RNA hybrids.
Feature
• Source : Bacteriophage T4
Application
• Joining of double-stranded DNA with cohesive or blunt end • Inversed PCR product
• Joining of linker or adaptors to blunt-ended DNA • Sub-cloning
• Repairing nicks in duplex DNA, RNA or DNA-RNA hybrids • Cloning of restricted DNA fragments into a plasmid
Product Information
BioFACT™ T4 DNA Ligase [DL101-40k]
▶Contents
T4 DNA Ligase (400 U/㎕),
10X Ligase Bu er
Maker Cat.No. Product Size
BIOFACT DL101-40k BioFACT™ T4 DNA Ligase 40,000 U
198 BIOFACT CATALOGUE
