Page 195 - 2022-23 BIOFACT Catalogue

DNA / RNA Preparation

- Know-how for high yield

1. Prepare 80% EtOH in a WB bottle and make fresh each time.
2. Place the gel block into UB Bu er and mix them thoroughly by pipetting.
3. EtOH should be completely removed before elution step.
4. Choose the appropriate method (Dimer removal / High yield) according to each puri cation purpose.
(Please perform high yield method in case of gel extraction.)
* In order to increase the e ciency during gel extraction method,
- It is recommended to use high quality agarose.
- Place the gel block into UB Bu er and dissolve them thoroughly at 50~60℃ and perform column binding.
- dissolve them by adding more than 5 times of UB bu er for puri cation at high percentage agarose gels.
5. Please use the kit prior to the expiration date.
6. Isolation e ciency can be increased when using EB bu er after pre-heating treatment for 10mins at 50℃ during DNA elution step.
(Especially, in case of long fragment)
7. When washing using NWB without ethanol, extraction time can be shortened by skipping column-idle step.

Product Information

HiGene™ Gel & PCR Purification System [GP104-100]

▶Contents
UB
EB
Help B
NWB
WB Bottle
Spin Column & Collection Tube

Reference
1. WIN, Theint Theint, et al. Monitoring the microbial community shift throughout the shock changes of hydraulic retention time in an anaerobic moving bed mem-
brane bioreactor. Bioresource technology, 2016, 202: 125-132
2. LEE, Seung-Yeol, et al. Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR. The plant pathology
journal, 2014, 30.4: 445.

Maker Cat.No. Product Size
GP104-100 HiGene™ Gel & PCR Puri cation System 100 prep
BIOFACT
GP104-200 HiGene™ Gel & PCR Puri cation System 200 prep
It is available to purchase individually in case of Spin Column & Collection Tube separately. Please contact us for more details.

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BIOFACT CATALOGUE