Page 201 - 2022-23 BIOFACT Catalogue

Ligase & Electrophoresis Reagent

EcoDye™

Nucleic Acid Staining Solution

Safer Alternative to EtBr for Detecting Nucleic Acid

EcoDye™ Nucleic Acid Staining Solution is a stable and sensitive DNA/RNA uorescent dye. It is Alternatives to EtBr non-mutagenic
and non-cytotoxic and can be used instead of Ethidium Bromide(EtBr) which is highly toxic and used for staining dsDNA, ssDNA or
RNA in agarose gels. EcoDye™ Nucleic Acid Staining Solution forms a complex through combination with DNA and can be detected
by UV imaging or LED system.

Feature
• Alternatives to EtBr • Detection of dsDNA, ssDNA & RNA
• High sensitivity • Various stability tests passed
• Non-toxic, non-mutagenic and non-carcinogenic (Ames test, Micronucleus test & In vitro mammalian
cell chromosomal aberration test)
• Excitation 290 – 320 nm, Emission 525 – 530 nm

Comparison of activity on various detection methods

Gel - Doc UltraSlim Transilluminator BlueView
(Wavelength : 312nm, UV) (Wavelength : 302nm, UV) (Wavelength : 470nm, Visible light)

1.5% Agarose Gel Staining (Pre-casting Step)

DNA / RNA
Sample Loading

Mixing 1 X TAE Mixing EcoDye TM Agarose gel
Bu er 100 ㎖ + Heating 3~5 mins Nucleic Acid Staining
Agarose 1.5 g Solution 10 ㎕ solution casting

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BIOFACT CATALOGUE