Page 202 - 2022-23 BIOFACT Catalogue

QC DATA
- Comparison of uorescence intensity between EcoDye™ Nucleic Acid Staining Solution, the other company's
EtBr alternative and EtBr
<Multiplex PCR>

EtBr EcoDye™ Company A
M 1 2 3 4 5 6 M 1 2 3 4 5 6 M 1 2 3 4 5 6

M : Rice marker 1 : PCR product 12 ㎕ 2 : PCR product 10 ㎕ 3 : PCR product 8 ㎕ 4 : PCR product 6 ㎕ 5 : PCR product 4 ㎕ 6 : PCR product 2 ㎕
Multiplex(Rice) PCR product 1.0X TAE buffer 3% Agarose gel (10 ㎕ mix /100 ㎖ agarose gel solution)

- Activity test of Ecodye in agarose gel by the passage of observation time

On the day After 1 week After 2 weeks After 3 weeks
1 1 1 2 3 4 5 1 2 3 4 5 1 2 3 4 5

1 : 1kb DNA Ladder 5㎕ 2 : 1kb DNA Ladder 4㎕ 3 : 1kb DNA Ladder 3㎕ 4 : 1kb DNA Ladder 2㎕ 5 : 1kb DNA Ladder 1㎕
1% Agarose gel

- Comparison of band migration shifts of EtBr alternative between EcoDye™ Nucleic Acid Staining Solution and other
EtBr alternatives

BIOFACT Company A Company B Company C

M 1 2 3 4 5 6 M M 1 2 3 4 5 6 M M 1 2 3 4 5 6 M M 1 2 3 4 5 6 M

M : 1 kb DNA Ladder 1-6: Template volume
1 : Human gDNA 100 ng 2 : Human gDNA 50 ng 3 : Human gDNA 25 ng 4 : Human gDNA 12.5 ng 5 : Human gDNA 6 ng 6 : Human gDNA 3 ng
Multiplex PCR using zincfinger pimer 3% Agarose gel, 7 ㎕ loading

202 BIOFACT CATALOGUE