Page 200 - 2022-23 BIOFACT Catalogue

- Comparison of over ow test when making 3% agarose gel between gel-rose™ Agarose and other companies' agaroses
(A) (B) (C)

Over ow has been showed in case of agaroses of
company A and company B
BIOFACT gel-rose™ Company A Company B

QC DATA

- Comparison of cloning e ciency on various agarose purity between gel-rose™ Agarose and other companies' agaroses

Cloning and colony PCR were performed to check target insert DNA after gel extraction step of PCR product using various agaroses.

< PCR Product 500 bp > < PCR Product 1.5kb >
(A) Agarose of BIOFACT gel-rose™ (A) Agarose of BIOFACT gel-rose™

(B) Agarose of company A (B) Agarose of company A

(C) Agarose of company B (C) Agarose of company B

결과 : Gel extraction에 사용되는 Agarose의 purity가 Cloning 효율에 영향을 미치는 것을 확인
- Conclusion: It has been con rmed that purity of agarose used in gel extraction step a ected the e ciency of cloning.
500 bp 1.5 kb 평균
500 bp 1.5 kb Average
BIOFACT
13 / 15
BIOFACT 13 / 15 (ea) 10/ 15 (ea) 10 / 15 76% 76 %
10 / 15
Company A 10 / 15 (ea) 6 / 15 (ea) 6 / 15 53% 53 %
A 사
Company B 9 / 15 (ea) 3 / 15 (ea) 3 /15 40% 40 %
9 / 15
B 사

200 BIOFACT CATALOGUE