Page 48 - 2022-23 BIOFACT Catalogue

LyoFACT™ All in one Pre-Mix

Lyophilized Typed PCR Pre-Mix Including Primers

LyoFACT™ All in one PCR Pre-Mix includes primers on the kit and it can conveniently be used by adding template RNA & RNase free
water only. It reduces and minimizes the possibility of contamination & varation during PCR ampli cation.

Feature
• Source : Thermus aquaticus • 12-13 bp error / 10 bp
6
• 5' → 3' exonuclease activity : Yes • A-tailing : Yes
• 3' → 5' exonuclease activity (Fidelity) : No • Pre-Mix with primers on the package
• IT102A-096 [M13(-20)F,M13(-20)R] IT102B-096 [M13(-47)F,M13(-48)R] • High solubility
/ IT103-096 [E.coli MLST Primer Set] • Minimization of temperature restriction

Application
• Routine PCR • TA cloning
• PCR screening • Colony PCR

QC DATA

Plasmid & colony (IT102A-096, IT12B-096) PCR test

<Plasmid DNA, 500 bp> <Plasmid DNA, 1 kb>
Company A – Lyophilized Type [IT102A-096]-Lyophilized Type Company A – Lyophilized Type [IT102B-096]-Lyophilized Type
M A B C D E F NTC M A B C D E F NTC M M A B C D E F NTC M A B C D E F NTC M

[IT102A-096] LyoFACT™ All in one Colony PCR Pre-Mix (M13-20 primer) [IT102B-096] LyoFACT™ All in one Colony PCR Pre-Mix (M13-47 primer)
A : 10 0 (1 ng) B : 10 -1 C : 10 -2 D : 10 -3 E : 10 -4 F : 10 -5 A : 10 0 (1 ng) B : 10 -1 C : 10 -2 D : 10 -3 E : 10 -4 F : 10 -5
NTC : No-Template Control M. 1Kb DNA Ladder NTC : No-Template Control M. 1Kb DNA Ladder
<Plasmid DNA, 3kb> <Colony PCR>
Company A – Lyophilized Type [IT102A-096]-Lyophilized Type [IT102B-096] - Lyophilized Type
M A B C D E F NTC M A B C D E F NTC M M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 M NTC

[IT102A-096] LyoFACT™ All in one Colony PCR Pre-Mix (M13-20 primer) [IT102B-096] LyoFACT™ All in one Colony PCR Pre-Mix (M13-47 primer)
A : 10 0 (1 ng) B : 10 -1 C : 10 -2 D : 10 -3 E : 10 -4 F : 10 -5 NTC : No-Template Control
NTC : No-Template Control M. 1Kb DNA Ladder

48 BIOFACT CATALOGUE