Page 53 - 2022-23 BIOFACT Catalogue

DNA / RNA Amplification Real-Time PCR Enzyme

Fluorescence
Real-Time PCR Work flow
Detection System

• BioFACT™ RT Series (p.102)
DNA RNA

OneStep
qRT-PCR

Intercalating dye Probe
• BioFACT™ Real-Time PCR Series
(For Intercalating Dye) (p.56)

SFCgreen® I Dye SYBR® Green I Dye EvaGreen™ Dye TaqMan® Probe

• 2X Real-Time PCR Master Mix • 2X Real-Time PCR Master Mix • Real-Time PCR • 2X Multi-Star Real-Time PCR Master Mix
Standard Type
• 2X Real-Time PCR Pre-Mix • 2X Real-Time PCR Pre-Mix • OneStep Multi-Star qRT-PCR Series
• 2X Real-Time PCR
Master Mix

SFCgreen® I Dye SYBR® Green I Dye EvaGreen™ Dye TaqMan® Probe

Sensitivity **** ** *** ****
Specificity * * * ****
Fluorescent signal ***** ** **** **
Multiplex PCR Impossible Impossible Impossible Possible
Melting curve required required required Not required
Primer & Probe design Easy Easy Easy Di cult

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BIOFACT CATALOGUE