Page 78 - 2022-23 BIOFACT Catalogue

BioFACT™

F-Star Taq DNA Polymerase

Optimal Taq DNA Polymerase for Less Than 3kb Speci c PCR

BioFACT™ F-Star Taq DNA Polymerase is designed to be highly speci c to the target gene through chemical-mediated Hot Start
system. It has similar features with BioFACT™ H-Star Taq DNA Polymerase and especially it is suitable to amplify DNA fragments larger
than 1kb. In case of chemical-mediated Hot Start PCR enzymes, its ampli cation e ciency and productivity may be lower than gen-
eral Taq DNA Polymerase. In this case, it can be supplemented by increasing the cycle.

Feature
• Source : Thermus aquaticus • A-tailing : Yes
• 5' → 3' exonuclease activity : Yes • Hot Start activity (Chemical-mediated Hot Start)
• 3' → 5' exonuclease activity ( delity) : No • All the advantages of Hot Start PCR enzyme and general Taq DNA Polymerase
• Ampli cation size : < 3 kb PCR • Error rate : 12 - 13bp error / 10 bp
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Application
• Routine PCR • TA Cloning
• SNP / Genotyping • Suitable for UDG system

QC DATA

- Comparison of PCR activity between BioFACT™ F-Star Taq DNA Polymerase and BioFACT™ Taq DNA Polymerase Various enzyme units

BioFACT™ Taq BioFACT™ F-Star
M 1 2 3 4 5 M M 1 2 3 4 5 M
Template : Human genomic DNA
Target gene : β-globin
PCR conditions : AT 65℃, 35 cycles
M : 1kb DNA Ladder
1 : 2.5 U 2 : 1.25 U 3 : 0.62 U 4 : 0.31 U 5 : 0.15 U

- Comparison of PCR activity between BioFACT™ F-Star Taq DNA Polymerase and BioFACT™ Taq DNA Polymerase Various
concentrations of Band Helper™
BioFACT™ Taq BioFACT™ F-Star
M 1 2 3 1 2 3 M
Template : Soybean
Target gene : IFS gene, 2 Band Amplification
PCR Conditions : AT 60℃, 35 cycles
M : 1kb DNA Ladder
1 : 0X Band Helper™ 2 : 1X Band Helper™ 3 : 2X Band Helper™

78 BIOFACT CATALOGUE