Page 82 - 2022-23 BIOFACT Catalogue

BioFACT™

Taq DNA Polymerase

The Most Common PCR Enzyme, Taq DNA Polymerase

BioFACT™ Taq DNA Polymerase is a thermostable DNA polymerase from Thermus aquaticus that possesses 5' to 3' polymerase activity. It
is the enzyme most widely used in various PCR-based techniques from routine PCR applications such as genotyping, RT-PCR for diagnosis
and colony PCR to demanding PCR applications.

Feature
• Source : Thermus aquaticus • Hot Start activity : No
• 5' → 3' exonuclease activity : Yes • A-tailing : Yes
• 3' → 5' exonuclease activity ( delity) : No • Error rate : 12 - 13 bp error / 10 bp
6
• Ampli cation size : < 5 kb PCR

Application
• Routine PCR • Colony PCR
• PCR for diagnosis • Suitable for UDG System
• TA Cloning

QC DATA
- Comparison of amplification efficiency according to various PCR fragment sizes

BIOFACT Company A Company B Company C Company D
M A B C D E M A B C D E M A B C D E M A B C D E M A B C D E M
Target : Human genomic DNA 50 ng
Gene : β-Globin
PCR Condition : 60°C, 35 cycles
M : 1kb DNA Ladder
A : 0.5 kb B : 1 kb C : 2 kb D : 3.6 kb E : 5 kb

- Comparison of amplification efficiency according - Comparison of activity test according
to various template concentrations to various enzyme units
M A B C D E F M M A B C D E M
BIOFACT BIOFACT
Company A Company A

Company B Company B

Company C Company C
Company D
Company D
Target : Human genomic DNA 10 ng Target : Human genomic DNA 10 ng
PCR Condition : 60°C, 35 cycles PCR Condition : 60°C, 35 cycles
M : 1kb DNA Ladder M : 1kb DNA Ladder
A : 50 ng B : 10 ng C : 2 ng D : 0.4 ng E : 0.08 ng F : 0.016 ng A : 2.5U B : 1.25U C : 0.625U D : 0.313U E : 0.156U

82 BIOFACT CATALOGUE