Page 80 - 2022-23 BIOFACT Catalogue

BioFACT™

Thumb Taq DNA Polymerase

Taq DNA polymerase with Increased PCR e ciency

BioFACT™ Thumb Taq DNA Polymerase is a widely used polymerase product most commonly used in the traditional PCR with applica-
tions in general PCR such as colony PCR as well as other various types of PCR methods for diagnosis and testing. Due to high ampli ca-
tion e ciency compared to Taq DNA polymerase, a large amount of PCR products can be obtained.

Feature
• Source : Thermus aquaticus • A-tailing : Yes
• 5' → 3' exonuclease activity : Yes • Hot Start activity : No
6
• 3' → 5' exonuclease activity ( delity) : No • Error rate : 12 - 13bp error / 10 bp
• Ampli cation size : < 5 kb PCR

Application
• Routine PCR • TA Cloning
• SNP / Genotyping

QC DATA
- Comparison of enzyme activity by various enzyme units between BioFACT™ Taq and BioFACT™ Thumb Taq
< AP DNA endonuclease gene Human 1 Kb > < β-Globin gene Human 3.6 Kb >
BioFACT™ Taq BioFACT™ Thumb Taq BioFACT™ Taq BioFACT™ Thumb Taq
M 1 2 3 4 5 6 M 1 2 3 4 5 6 M 1 2 3 4 5 6 M 1 2 3 4 5 6 Human Genomic DNA
1 2 3 4 5 6
1 2 3 4 5 6
1 2 3 4 5 6
1 2 3 4 5 6
10 ng, 35 cycles
M : 1kb DNA Ladder
1: 2 unit
1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 2: 1 unit
1 2 3 4 5 6
1 2 3 4 5 6
1 2 3 4 5 6
1 2 3 4 5 6
3: 0.5 unit
4: 0.25 unit
5: 0.125 unit
6: 0.0625 unit
- Comparison of amplification efficiency by various template concentrations between BioFACT™ Taq and BioFACT™ Thumb Taq
1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6
< AP DNA endonuclease gene Human 1 Kb > < β-Globin gene Human 3.6 Kb >
BioFACT™ Taq BioFACT™ Thumb Taq BioFACT™ Taq BioFACT™ Thumb Taq
M 1 2 3 4 5 6 M 1 2 3 4 5 6 M 1 2 3 4 5 6 M 1 2 3 4 5 6 Enzyme 0.625 unit,
35 cycles
M : 1kb DNA Ladder
1: 50 ng
2: 10 ng
3: 2 ng
4: 0.4 ng
5: 0.08 ng
6: 0.016 ng

80 BIOFACT CATALOGUE