Page 86 - 2022-23 BIOFACT Catalogue
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BioFACT™


                  A-Star Taq DNA Polymerase


                  Antibody-Mediated Hot Start PCR Enzyme






                  BioFACT™ A-Star Taq DNA Polymerase, isolated from Thermus aquaticus and expressed in E.coli through a simple and modi ed puri ca-
                  tion procedure, is Hot Start PCR enzyme which is combined with anti-Taq monoclonal antibody that inhibits polymerase activity until it
                  is heat denatured. It has shorter pre-denaturation time than chemical-modi ed Hot Start PCR enzyme and it also prevents non speci c
                  ampli cation and primer-dimer formation. BioFACT™ A-Star Taq DNA Polymerase is highly suitable for ampli cation reactions involving
                  low-copy targets, complex genomic DNA or cDNA templates. Providing optional 5X Band Helper™ helps ampli cation of di cult PCR
                  caused by high GC content or complex structure of template.




                    Feature
                    • Source : Thermus aquaticus         • Hot Start activity (Antibody-mediated Hot Start) : Yes
                    • 5' →  3' exonuclease activity : Yes  • All the advantages of Hot Start PCR enzyme and general Taq DNA Polymerase
                    • 3' →  5' exonuclease activity ( delity) : No  • High speci city & minimization of primer-dimer formation
                    • Ampli cation size : < 5 kb PCR     • High activity & high sensitivity
                    • A-tailing : Yes                    • Easy PCR optimization



                    Application
                    • Routine PCR                        • Colony PCR
                    • PCR for diagnosis                  • Suitable for UDG system
                    • TA cloning

                    QC DATA

                  - Comparison of amplification efficiency according to various   -  Comparison of amplification efficiency according to vari-
                     enzyme units between BioFACT™ A-Star Taq DNA Polymerase       ous template concentrations between BioFACT™ A-Star
                     and BioFACT™ Taq DNA Polymerase                   Taq DNA Polymerase and BioFACT™ Taq DNA Polymerase

                           Taq               A-Star Taq                      Taq               A-Star Taq
                   M   A       B      C      D      E      F  M   A       B      C      D      E      F  M   A       B      C      D      E      F  M   A       B      C      D      E      F
              NF-κ B                                           NF-κ B
             (1.2 kb)                                          (1.2 kb)

             TNFR1                                             TNFR1

                  Target : Human genomic DNA 10 ng           PCR Condition : 60℃, 35 cycles  Target : Human genomic DNA                    PCR Condition : 60℃, 35 cycles
                  M : 1kb DNA Ladder                                M : 1kb DNA Ladder
                  A : 50 ng      B : 10 ng      C : 2 ng      D : 0.4 ng      E : 0.08 ng      F : 0.016 ng  A : 50 ng      B : 10 ng      C : 2 ng      D : 0.4 ng      E : 0.08 ng      F : 0.016 ng







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